Cyclin A is destroyed in prometaphase and can delay chromosome alignment and anaphase. J. Cell Biol. 153, 121–136 (2001). Google Scholar Yamano, H. APC/C: current understanding and future perspectives. F1000Res. https://doi.org/10.12688/f1000research.18582.1 (2019). Article PubMed Pub...
Metaphase II-arrested oocytes were collected from superovulated F1 female mice (C57Bl/6J × CBA) aged <10 weeks and cumulus cells were removed using hyaluronidase. Oocytes were enucleated in a droplet of M2 medium containing 5 μg ml−1 of cytochalasin B (CB) using a blunt ...
Karyokinesis, also known as mitosis, is divided into a series of phases—prophase, prometaphase, metaphase, anaphase, and telophase—that result in the division of the cell nucleus ((Figure)). Figure 2.Karyokinesis (or mitosis) is divided into five stages—prophase, prometaphase, metaphase, a...
Interphase (actually, this is not part of mitosis itself) Prophase Metaphase Anapha 3、se TelophaseTHE STEPS OF MITOSISOnion root tipWhitefish blastulaInterphase Nuclear envelop intact Chromosomes duplicated but not apparent 2 nucleoli visible in onion root tip Nucleoli not visible in whitefish blastula...
Post-mitotic incubation of cells arrested in metaphase by 6h incubation with 10mM DMAA showed that the incidence of multinucleated cells increased conversely with a rapid decrease in metaphase cells. This suggests that metaphase-arrested cells can escape from metaphase, resulting in the appearance of ...
After prophase I,the meiotic cell enters metaphase I.During this phase,the nuclear membrane breaks down,allowing microtubules access to the chromosomes.Still joined at their crossover regions in tetrads,the homologous pairs of chromosomes,with one maternal and one paternal chromosome in each pair,...
The comparison between s-SCF levels and clinical outcomes showed a statistically significant correlation between both the number of metaphase II (MII) oocytes retrieved and the embryos obtained after fertilization. Cases with at least 3 MII oocytes showed s-SCF values >800 pg/mL, 2 MII oocytes ...
(prometaphase). At this point, kinetochores that are unattached to the mitotic spindles generate a “wait” signal that delays the onset of anaphase until all chromosomes are properly attached and aligned. This signal is also called mitotic checkpoint or spindle assembly checkpoint (SAC), which ...
The developmental ability of blastomeres of late 2-cell stage embryos fused to chemically enucleated oocytes of different ages or cytoplasts from different sources was also examined in vitro. Metaphase I oocytes cultured in different concentrations of ETO-CHXM (10-50 micrograms/ml/each) extruded ...
Fluorescence in situ hybridization (FISH) analysis of normal human metaphase spreads using a 100 kb nonchimeric PAC clone (isolated with a probe [1.0 EH] representing non-IGH sequences) identified the partner chromosomal locus as band 1q21 Figure 1, Figure 5. Download: Download high-res image...