Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Production of site-specific antibody–drug conjugates using optimized non-natural amino acids in a cell-free expression system Bioconjug Chem, 25 (2014), pp. 351-361 CrossrefView in ScopusGoogle Scholar This paper demonstrates the production of an antibody-drug conjugate (ADC) to near homogeneity...
Cell-free protein synthesis (CFPS) platforms can be used for rapid and flexible expression of proteins. The use of CFPS platforms from mammalian, specifically Chinese hamster ovary (CHO) cells, offers the possibility of a rapid prototyping platform for recombinant protein production with the capabilit...
Althoughin vitroexpression is not practical for commercial large-scale recombinant protein production, it has a variety of features that make it considerably more useful and flexible for many research applications. Applications for which ...
Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase Christin Slomka, Georg Paris Späth, Phillip Lemke, Marc Skoupi, Christof M. Niemeyer, Christoph Syldatk & Jens Rudat AMB ...
One possibility to increase the throughput in AMP production is to switch from chemical synthesis to DNA-based bioproduction methods. However, heterologous expression of AMPs in microorganisms, such asE. coli, features several disadvantages: (i) it is time- and labor-intensive, (ii) it requires ...
Endo Y, Sawasaki T: High-throughput, genome-scale protein production method based on the wheat germ cell-free expression system. Biotechnol Adv. 2003, 21 (8): 695-713. 10.1016/S0734-9750(03)00105-8. Article CAS Google Scholar Bradford MM: A rapid and sensitive method for the quantitati...
During male meiosis, the Y chromosome can form perfect pairing with the X chromosome. However, it is unclear whether mammalian Female germline stem cells (FGSCs) without a Y chromosome can transdifferentiate into functional haploid spermatid-like cells (
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways Article Open access 27 November 2019 A human expression system based on HEK293 for the stable production of recombinant erythropoietin Article Open access 14 November 2019 Introduction...
Sign up for alerts RSS feed nature nature communications articles article Article Open access Published: 04 February 2019 A cell-free platform for the prenylation of natural products and application to cannabinoid production Meaghan A. Valliere, Tyler P. Korman, Nicholas B. Woodall, Gregory A...