Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
One possibility to increase the throughput in AMP production is to switch from chemical synthesis to DNA-based bioproduction methods. However, heterologous expression of AMPs in microorganisms, such asE. coli, features several disadvantages: (i) it is time- and labor-intensive, (ii) it requires ...
There’re two categories of cell-free expression systems. So far, prokaryoticin vitrotranslation cell-free systems have been the hotspot of interest. Recently, eukaryotic cell-free systems have enriched the antibody production pipeline, as the reactions can mimic the natural pathway of antibody synthe...
Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system Mabs, 7 (2015), pp. 231-242 CrossrefView in ScopusGoogle Scholar 27 Y. Lu, J.P. Welsh, J.R. Swartz Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially...
Adipose-derived mesenchymal stem cells reduce MMP-1 expression in UV-irradiated human dermal fibroblasts: therapeutic potential in skin wrinkling. Biosci Biotechnol Biochem. 2015;79(6):919–25. PubMed CAS Google Scholar Guo S, Wang T, Zhang S, Chen P, Cao Z, Lian W, et al. Adipose-...
Different GOI's will likely have unpredictable effects on expression of FI-Zeo and thus the capacity of a transfected cell to survive zeocin selection, however, by having the flexibility of three different zeocin selection strengths in the STEP®1, STEP®2 and STEP®3 vectors, production ...
expression of genes in alternate pathways that branch off the pathway of interest, and this can be concomitantly administered, with care taken to ensure that these manipulations do not undermine cellular viability and robustness. Alleviating chemical bottlenecks that might hamper the production of the ...
Co-expression network was constructed using WGCNA. Module- and hub-based methods were adopted to predict the functions of lncRNAs. Results A total of 77,382 cells during the differentiation process of hESCs toward pancreatic progenitors were sequenced. According to the single-cell map, the cells ...
抗体药物开发是一个复杂而精密的过程,而细胞株开发则是生物制药过程中至关重要的一步,它涉及宿主细胞的选择、表达载体工程、转染与筛选等关键步骤。其目的是构建一个稳定、高效且符合生产的细胞株,这将在很大程度上决定药物开发的效率和成本。 本文分享基于CHO细胞株的抗体药物开发策略[1],提高细胞株开发效率并实现高...
Protein expression and purification Expression of all enzymes except FDH was performed by growing in 1 L of LB auto-induction media with 50 µg/mL kanamycin at 37 °C for 20 h. Auto-induction media was prepared by adding 0.5 g glucose and 2 g lactose mixture into Miller’s formula LB...