Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Innovative Applications for Cell-Free ExpressionRobin Hurst
Large-scale Media Production in cGMP Facility Available in Standard Bottles & Flexible Bags Get Started News & Events Xeno-Free Media Specials Choose Cell Applications Xeno-Free & Chemically-defined Media for your Research and Drug Discovery.Learn More ...
One possibility to increase the throughput in AMP production is to switch from chemical synthesis to DNA-based bioproduction methods. However, heterologous expression of AMPs in microorganisms, such asE. coli, features several disadvantages: (i) it is time- and labor-intensive, (ii) it requires ...
Although in vitro expression is not practical for commercial large-scale recombinant protein production, it has a variety of features that make it considerably more useful and flexible for many research applications. Applications for whi...
Cell-free production of G protein-coupled receptors for functional and structural studies J. Struct. Biol., 158 (2007), pp. 482-493 View in ScopusGoogle Scholar [13] G. Ishihara, M. Goto, M. Saeki, K. Ito, T. Hori, T. Kigawa, M. Shirouzu, S. Yokoyama Expression of G protein co...
The use of cell-free systems for labeling applications in NMR work has become more popular and has been expanded to the labeling of membrane proteins (Sobhanifar et al., 2010). Cell-free expression systems allow the direct production of membrane proteins in membrane-mimetic environments that may...
1. Marlitt, S.;et al. Cell-free synthesis meets antibody production: A review.Antibodies. 2015, 4: 12-33. 2. Xu, Y.;et al. Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system.Mabs. 2015, 7(1): 231-242. ...
Moreover, the expression of immune checkpoints, such as CD137 (4-1BB), inducible co-stimulator (ICOS), T cell immunoglobulin and mucin domain 3 (TIM-3) changed in the tumor microenvironment could affect tumor progression [13]. The understanding of ligand-receptor interaction is the foundation...
Toward a cell-free hydantoinase process: screening for expression optimization and one-step purification as well as immobilization of hydantoinase and carbamoylase Christin Slomka, Georg Paris Späth, Phillip Lemke, Marc Skoupi, Christof M. Niemeyer, Christoph Syldatk & Jens Rudat AMB ...