Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Because protein synthesis occurs in cell lysates rather than within cultured cells, the method is also called cell-free protein expression. Cell-free protein production can be accomplished with several kinds and species of cell extract...
We have optimized cell-free expression protocols for the human endothelin A and endothelin B receptors by implementing co-translational association approaches of the synthesized proteins with supplied liposomes or nanodiscs. Efficiency of membrane association and ligand binding properties of the receptors ...
Cell-free protein synthesis (CFPS) platforms can be used for rapid and flexible expression of proteins. The use of CFPS platforms from mammalian, specifically Chinese hamster ovary (CHO) cells, offers the possibility of a rapid prototyping platform for recombinant protein production with the capabilit...
Translating heterologous proteins places significant burden on host cells, consuming expression resources leading to slower cell growth and productivity. Yet predicting the cost of protein production for any given gene is a major challenge, as multiple p
Single-cell technique occurring withDifferential Expression Analysis Protocol Springer Protocols(2019) Trajectory Algorithms to Infer Stem Cell Fate Decisions Edroaldo Lummertz da Rocha,Mohan Malleshaiah Single-cell trajectory analysis is an active research area in single-cell genomics aiming at developing...
The temperature inducible expression system, based on the pL and/or pR phage lambda promoters regulated by the thermolabile cI857 repressor has been widely use to produce recombinant proteins in prokariotic cells. In this expression system, induction of
ComboSC utilizes tumor cell expression data from the CCLE database [46, 47] and corresponding drug response data for CCLE expression data from GDSC [42, 43] for cell type-specific drug response prediction. Based on the highest similarity score of the CCLE cell line to each cell group, combo...
The gene knockdown of NEBL inhibits mineralized nodule formation, alkaline phosphatase activity, and the expression of bone marker genes, indicating that it can be used as a functional qualitative HAOB marker in the development of bone regenerative therapeutics with these cells. However, further ...
Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system Mabs, 7 (2015), pp. 231-242 CrossrefView in ScopusGoogle Scholar 27 Y. Lu, J.P. Welsh, J.R. Swartz Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially...