3b, c, g). Both MIF and MK were also upregulated on protein level in NB tumors and cell lines compared to controls, and receptor expression was high on resting and activated monocytes (Fig. 3h, i). To experimentally test the interactions of NB with myeloid cells, we measured secreted ...
5d, the OCR was lower in O-ADSCs than in L-ADSCs. Comparable results were observed for ATP production in the supernatant in O-ADSCs. We also measured the levels of enzymes involved in the glycolytic pathway (HK1, HK2, PFKP, and LDHA) and TCA cycle (CS, DLST, SDHA, and FUMA) by...
Citrate, a substance being related to de novo fatty acid synthesis and tricarboxylic acid (TCA) cycle, has a pivotal role in cell survival. However, the molecular mechanisms that regulate intracellular citrate in triple-negative breast cancer (TNBC), especially under hypoxic condition, remain poorly...
Isocitrate dehydrogenase (IDH), an enzyme of the TCA cycle, catalyses the oxidative decarboxylation of isocitrate to produce α-ketoglutarate; during this reaction it produces also a molecule of NADH, essential to feed ETC. Rises in ATP/ADP and NADH/NAD+ ratios inhibit the activity of the enzy...
复旦大学附属肿瘤医院雷群英课题组在Nature Cell Biology杂志上发表了题为 “BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma” 的研究论文。作者揭示了BCAA - …
Targeting cancer stem cell OXPHOS with tailored ruthenium complexes as a new anti-cancer strategy Sonia Alcalá, Lara Villarino, Laura Ruiz-Cañas, José R. Couceiro, Miguel Martínez-Calvo, Adrián Palencia-Campos, Diego Navarro, Pablo Cabezas-Sainz, Iker Rodriguez-Arabaolaza, Alfonso...
PC- PUFA2s处理,包括PC(22:6, 22:6)和PC(20:4, 20:4),与PC- PUFA1或给药处理相比,显著降低了线粒体膜极化,罗达明123标记减少(图5B和S5A)。然后他们测定耗氧量(OCR)来反映线粒体呼吸活动。PC(22:6, 22:6)治疗显著抑制OCR,与fer-1联合治疗后OCR部分恢复(图5C)。RSL3不影响OCR,这与之前的报道...
Douglas Melton (Department of Stem Cell and Regenerative Biology, Harvard University). H3083 iPSC line was reprogrammed from a 59-year-old healthy donor (male)-derived peripheral blood mononuclear cells in the laboratory of Dr. Jean Jieun Kim (Baylor College of Medicine). iPSCs were cultured ...
depletes certain serum metabolites, and CD5KO T cells have higher levels of several metabolites. Transcriptomic analysis identified several upregulated metabolic genes in CD5KO T cells. Bioinformatic analysis identified glycolysis and the TCA cycle as metabolic pathways promoted by CD5 removal. ...
In general, a small proportion of ATP synthesis is accomplished by glycolysis in the cytoplasm, whereas the majority is produced in mitochondria by intimate exchange between the TCA cycle and OXPHOS. Mitochondrial fatty acid β-oxidation (FAO) provides an additional source for ATP generation. The ...