aSchematic showing construction of the cardiomyocyte-specificKlf7-knockout mouse model.b,cKlf7deficiency upregulatedPfkland downregulatedAcadlat the mRNA and protein level, as assessed by qRT-PCR (b, WT,n = 3 biologically independent samples, KO,n = 4 biologically independent samples;c,...
of other maturation-related parameters. Although T623 can terminally reduce cell-cycle active cells in a dose-dependent manner (Supplementary Fig.4g and h), higher concentrations do not lead to a more mature phenotype, suggesting that T623-induced maturation is not mediated by cell cycle arrest....
The citric acid cycle was also significantly impacted (Figure 2D), which, when coupled with our transcriptomics data and the accumulation of acyl-carnitines and BCAAs, is suggestive of an overall decrease in oxidative metabolism. Significant enrichment in pathways associated with lipid biosynthesis (...
In vivo, AAV9-mediated overexpression of VDAC1 in myofibroblasts significantly alleviated transverse aortic constriction (TAC)-induced cardiac fibrosis and rescued cardiac function in mice. Finally, we treated mice with the VDAC1-derived R-Tf-D-LP4 peptide, and the results showed that R-Tf-D-...
Semaglutide, a glucagon-like peptide-1 receptor agonist, is clinically used as a glucose-lowering and weight loss medication due to its effects on energy metabolism. In heart failure, energy production is impaired due to altered mitochondrial function an
Heart organoids are comparable to age-matched human fetal cardiac tissues at the transcriptomic, structural, and cellular level. They develop sophisticated internal chambers with well-organized multi-lineage cardiac cell types, recapitulate heart field formation and atrioventricular specification, develop a ...
Given the potential for myocardial stem cell transplantation as a promising treatment for heart failure, numerous clinical trials have been conducted and its usefulness has been clearly confirmed. However, the low rate of engraftment of transplanted cells has become a clinical problem, and this needs...
The serine/threonine kinase, PINK1, and the E3 ubiquitin ligase, Parkin, are known to facilitate LC3-dependent autophagosomal encasement and lysosomal clearance of dysfunctional mitochondria, and defects in this process contribute to a variety of cardiometabolic and neurological diseases. Although recent ...
increased Sirt3 level due to polymorphism in the gene promoter was linked to extended lifespan of humans, whereas another polymorphism in the Sirt3 gene led to decreased enzymatic activity of Sirt3, and was found to be associated with metabolic syndrome in humans, thus implicating a role of Si...
expression level ofαSMAmRNA was calculated. Two-tailedt-test with Welch’s correction was used for the statistical analysis (t = 3.212, df = 3.172). Data show the mean and the standard deviation (error bar) of technical triplicates from a representative experiment (a,d). *p ...