流式细胞仪检测凋亡和细胞周期等应用.ppt 热度: AnalysisofCellCyclebyFlowCytometry301 301 From:MethodsinMolecularBiology,vol.281:CheckpointControlsandCancer,Volume2: ActivationandRegulationProtocols Editedby:AxelH.Schönthal©HumanaPressInc.,Totowa,NJ ...
B, Cell-cycle analysis after 7 days of ev or sgKAT6A-2 transduction in MOLM-13 cells (n = 4 each group). We evaluated the role of KAT6A in MLL-r AML in more depth. Consistent with the proliferation phenotypes, we found that loss of KAT6A inducedcell-cycle arrest(Fig. 2B) and mo...
Why flow cytometry is ideal for cell cycle analysis When stained with a cell cycle reagent, DNA in the cells bind the dye stoichiometrically (in proportion to the amount of DNA present in each cell). The flow cytometric analysis of cell count versus linear flu...
Figure 1. Flow cytometry DNA content distribution in a cell cycle analysis assay. (A) Histogram of live Jurkat cells stained with Vybrant DyeCycle Violet stain showing DNA content distribution. G0/G1 and G2/M phase histogram peaks are sepa...
2014. Cell Cycle Analysis by Flow Cytometry. Hoboken, NJ: WileyDarzynkiewicz Z, Zhao H. 2014. Cell cycle analysis by flow cytometry.Z. Darzynkiewicz, H. Zhao, Cell Cycle Analysis by Flow Cytometry, eLS, John Wiley & Sons, Ltd., 2001...
Cell Cycle Analysis by Flow Cytometry Flow cytometry, the methodology that provides a way to measure physical and chemical attributes of individual cells rapidly and with high accuracy, is wide... Z Darynkiewicz,H Zhao - American Cancer Society 被引量: 0发表: 2014年 [Methods in Enzymology] ...
摘要: This presentation reports on the routine use of flow cytometric techniques in suspension cultures. As a model system an secreting hybridoma line was used to monitor (a specific ) and proliferative status as obtained by flow cytometry (FCM) and counting.关键词:...
Analysis of Cell Cycle by Flow Cytometry The cell cycle of a cell is tightly controlled by several regulators. Dysregulation of cell cycle can lead to uncontrolled cell division which is one of th... H Maadi,MH Soheilifar,Z Wang - 《Methods in Molecular Biology》 被引量: 0发表: 2022年 ...
流式细胞术pi染色进行细胞周期分析的原理是什么(Whatisthe principleofcellcycleanalysisbyflowcytometryPIstaining) ChineseJournaloflaboratorymedicine N$$,year! Mmonths VolumeN],No. DuringtheperiodofM 3:6; U ZES O5A,OED?; N$$!9=.! N],*=> M #-"#. Providereliablebasisforconditionmonitoring. The...
DNA Content and Cell Cycle Analysis by Flow Cytometry in Clinical Samples: Applications in Cancer F Spyratos - 《Nato Asi》 被引量: 0发表: 1993年 DNA flow cytometry in solid tumors: Practical aspects and clinical applications DNA flow cytometry in solid tumors, practical aspects and clinical ...