Why flow cytometry is ideal for cell cycle analysis When stained with a cell cycle reagent, DNA in the cells bind the dye stoichiometrically (in proportion to the amount of DNA present in each cell). ...
Flow-based cytometric analysis of cell cycle via simulated cell populations. PLoS Comput Biol 2010;6:e1000741.Brown, M. R., Summers, H. D., Rees, P., Smith, P. J., Chappell, S. C., & Errington, R. J. (2010). Flow-based cytometric analysis of cell cycle via simulated cell ...
Cell cycle analysis of apoptosis using flow cytometry. Using flow cytometry to analyze asynchronous and synchronized cell populations, this chapter describes a number of features of drug-induced apoptosis in cu... SW Sherwood,RT Schimke - 《Methods in Cell Biology》 被引量: 228发表: 1995年 Cell...
We present a new approach to the handling and interrogating of large flow cytometry data where cell status and function can be described, at the population level, by global descriptors such as distribution mean or co-efficient of variation experimental data. Here we link the ''real'' data to...
Analysis of cell cycle and DNA synthesis during myocytic differentiation by flowcytometry 来自 知网 喜欢 0 阅读量: 37 作者:RF Qin,GU Xiao Ming,FG Huang 摘要: AIM To investigate cell cycle and DNA synthesisduring myocytic differentiation. METHODS Murine C2C12 myoblasts were used in this ...
YeastCellCyclebyFlowCytometry Cold absolute ethanol. 0.5 M Na citrate stock (filtered), 50mM diluted stock. 10 mg/ml RNase A (Boil 10 mins, cool, filter and store at -20°C). 4 mg/ml Propidium iodide (PI) (filter and store in dark at -20°C)....
Successful completion of the cell cycle usually results in two identical daughter progeny. This process of generational doubling is termed proliferation and when it occurs in a regulated fashion the benefits range from driving embryonic development to mounting a successful immune response. However when ...
FlowJo provides a simple interface to performing fairly sophisticated DNA/Cell Cycle analysis. To launch the univariate cell cycle model click on the population of interest in the workspace, then select the Cell Cycle task from theBiology Band. The univariate model will appear by default as shown...
Cell cycle progression can be studied with computational models that allow to describe and predict its perturbation by agents as ionizing radiation or drugs. Such models can then be integrated in tools for pre-clinical/clinical use, e.g. to optimize kine
Modern sugarcane is an unusually complex heteroploid crop, and its genome comprises two or three subgenomes. To reduce the complexity of sugarcane genome research, the ploidy level and number of chromosomes can be reduced using flow chromosome sorting. H