p. R527C).bImmunostaining of lamin A/C (LMNA, green) in normal and MAD-patient derived dermal fibroblasts. DAPI, blue. Scale bar 10μm. The experiment was repeated three times with similar results.cImmunostaining of lamin A/C (LMNA, green), H3K9me3...
Cell viability and proliferation were measured using MTT and colony formation, and the cell cycle and apoptosis was analyszed by flow cytometry. The changes of downstream protein level and protein phosphorylation level were detected by western blot. Results: PPP5C was highly expressed in PCa tissue...
Moreover, Hoechst staining assay and flow cytometry were performed to assess the cell apoptosis rate. Western blotting was used to assess the protein expression levels of key factors associated with apoptosis, the Akt signaling pathway, and the epithelial-mesenchymal transition. A xenograft model was...
T cells in the anergic state show a block in the G1/S transition, and the cell cycle progression of anergic cells stimulated with IL-2 rescues them from anergy1,30,46. Therefore, Ndrg1 may be involved in both cell types in a G1/S transition blockade. However, the fact that Ndrg1...
Cell viability and proliferation were measured using MTT and colony formation, and the cell cycle and apoptosis was analyszed by flow cytometry. The changes of downstream protein level and protein phosphorylation level were detected by western blot. Results: PPP5C was highly expressed in PCa tissue...
(Promega) and random primers. Each reaction was performed in triplicate from two cDNA dilutions. The comparative threshold cycle (Ct) method was used to calculate the amplification factor as specified by the manufacturer. Human GADPH was used as an internal standard to normalize variations in RNA...
In response to different types and intensities of mechanical force, cells modulate their physical properties and adapt their plasma membrane (PM). Caveolae are PM nano-invaginations that contribute to mechanoadaptation, buffering tension changes. However
To further study the possible involvement of GTIN's anti-proliferation in the modulation of VSMCs cell-cycle, the effects of GTIN (1, 5, and 10 µM) on A7r5 cells were detected by flow cytometry. In concordance with this VSMCs phenotypic transition, comparing with starvation group, a ...
The cell cycle phase was calculated by FlowJo software (Becton-Dickinson, Franklin Lakes, NJ, USA). 4.5. Apoptosis After seeding overnight, drug treatments were performed as indicated in the figure legend. Subsequently, apoptosis of non-fixed cells was determined by annexin V/7AAD, Caspase-Glo...
2. Results 2.1. Overexpression of hXIAP in the Spinal Cord of CB57BL/6 Mice (CB57hXIAP+) Reduction in XIAP expression after SCI in CB57WT mice (Figure 1A,B) can be an important factor for the secondary neuronal death induced by the trauma to the spinal cord. To test this ...