Full size image We determined whether increased fibroblasts were recruited by epithelial cells in fibroadenomas. We isolated primary epithelial and fibroblast cells from patient fibroadenoma samples and paired normal tissues and set up an epithelial and fibroblast coculture system (Supplementary Fig.3f, g...
Cell isolation and culture Primary mouse stem Leydig cells isolation was performed according to a previous article15. The testes were dissected from Nes-GFP or C57BL/6 mice, and then we removed the tunica albuginea carefully from each testis and minced the testes into small pieces. The interst...
industrial company. We offer suggestions for a proactive union approach which can enhance the role of workers and their unions in the successful implementation of this new technology.doi:10.1016/S1474-6670(17)49565-6K. KnaussM.H. MatuszakIFAC Proceedings Volumes...
从古代炼金术士的长生灵药到现代科学的抗衰研究,人们始终尝试打破命运的桎梏。 自20 世纪 60 年代,细胞生物学家 Hayflick 提出“细胞增殖极限”理论以来,衰老细胞一直被认为处于“不可逆的周期阻滞”状态:一旦陷入这一状态,便如同被困于“时间牢狱”,不断走向衰老。衰老细胞通常伴随多种细胞因子表达的上调和细胞分泌能...
运动对健康的益处是多方面的,它能在许多器官中产生适应性反应,防止代谢性疾病和衰老。 2024年Cell运动代谢学术研讨会于5月5日‒7日在葡萄牙里斯本举行。本次研讨会汇聚运动生理学家、系统生物学家、昼夜节律生物学家、营养学专家以及其他研究健康和疾病能量管理等交叉领域...
Stock solutions of different fractions of methanolic solutions (1 mg/mL) were prepared in methanol, stored at -20 ℃, and then diluted as needed in cell culture medium and for other experiments. MTT was purchased from Sigma-Aldrich (St. Louis, MO, USA). Penicillin, streptomycin, Roswell Par...
中文名称:Anti-Mast Cell Chymase:肥大细胞类糜蛋白酶1抗体英文名称:Anti-Mast Cell Chymase 保存条件:保存在4℃(短期)或-20℃(长期)纯度规格:Anti-Mast Cell Chymase:肥大细胞类糜蛋白酶1抗体 产品类别:科研试剂 "Anti-Mast Cell Chymase:肥大细胞类糜蛋白酶1抗体 ...
2、与传统的aPD-1治疗相比,BiCE治疗对肿瘤和肿瘤引流淋巴结的免疫重编程具有独特而卓越的效果。 2024年1月18日,以色列Weizmann科学研究所的研究人员在Cell期刊发表了题为:Bispecific dendritic-T cell engager potentiates anti-tumor immunity的研究文章。
The culture was grown at 37 °C and 120 RPM to an optical density 600 (OD600) ~0.6 at which point the expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) at 25 °C O/N. The following day, cells were harvested and resuspended in lysis buffer (PBS...
and we discuss new opportunities for exploiting the latest advances in cell-based assay technologies in drug discovery, including induced pluripotent stem cells, three-dimensional (3D) co-culture and organ-on-a-chip systems, complemented by advances in single-cell imaging and gene editing technologies...