H. et al. ALIX and ESCRT-III coordinately control cytokinetic abscission during germline stem cell division in vivo. PLoS Genet. 11, e1004904 (2015). PubMed PubMed Central Google Scholar Iwamori, T. et al. TEX14 interacts with CEP55 to block cell abscission. Mol. Cell. Biol. 30, ...
create "stu",{NAME => "cf",BLOCKSIZE =>"65536"} 设置stu cf列族块大小为64K, 块大小是HBase的一个重要配置选项,默认块大小为64k,默认单位是字节,采用这种细粒度,目的是块操作时更加有效加载和缓存数据,对块大小的调整,主要取决于两点: 1. 用户平均读取数据的大小。 理论上讲,如果用户平均读取数据的大小...
TruStain FcX block (BioLegend, 422301) was added before mixing and incubating for 10 min on ice. Next, 1 µg of TotalSeqA antibody was diluted in cell staining buffer, and 50 µl of this antibody dilution was added to the blocked cells before incubation on ice for 30 min....
Gainor JF, Shaw AT (2013) The new kid on the block: RET in lung cancer. Cancer Discov 3(6):604–606. doi:10.1158/2159-8290.CD-13-0174 Article CAS PubMed Google Scholar Garrett JE, Tamir H, Kifor O, Simin RT, Rogers KV, Mithal A, Gagel RF, Brown EM (1995) Calcitonin-sec...
Flagellin is the main building block of the organs responsible for moving almost all flagellated bacteria and the primary ligand of receptors like FLS2 (Schuster and Khan1994). Flagellin perception depends on FLS2 heterodimerization with the LRR-RLK co-receptor BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED ...
Single cell suspensions were first incubated with FcR Blocking Reagent (Miltenyi Biotec) in staining buffer (2% FBS, 0.1% Na azide, in PBS) for 10 min at 4°C to block binding to Fc receptors. Then the cells were stained with proper antibodies or matched isotype controls in staining buffer...
[8]. Recent single molecule experiments show that the MRN complex slides along the dsDNA and activates ATM when MRN hits a road block, such as KU at the DNA ends [95]. Prior biochemical studies also suggest that MRN unwinds the DNA ends and activates ATM via the ssDNA region [14]. ...
We chose Dynasore (a specific inhibitor of dynamin [DNM] uanosine triposphate hydrolase [GTPase]) (Nnah et al., 2018), which is supposed to block the endocytosis process, to dissect the role of accumulated late endosome/lysosome in Vps33B-deficient cells. Dynasore treatment lowered the LAMP...
Lazar DF, Wiese RJ, Brady MJ, Mastick CC, Waters SB, Yamauchi K, Pessin JE, Cuatrecasas P, Saltiel AR: Mitogen-activated protein kinase kinase inhibition does not block the stimulation of glucose utilization by insulin. J Biol Chem. 1995, 270: 20801-20807. 10.1074/jbc.270.35.20801. Artic...
Abdel-Azim, H., Elshoury, A., Mahadeo, K. M., Parkman, R. & Kapoor, N. Humoral immune reconstitution kinetics after allogeneic hematopoietic stem cell transplantation in children: a maturation block of IgM memory B cells may lead to impaired antibody immune reconstitution. Biol. Blood Marrow...