we tracked GFP+MZ B cells in the first 18 h after intravenous transfer of splenocytes. Compared to the total number of MZ B cells that were injected, most cells were lost from the blood within 10 min, and only ~0.25% could be recovered 1 h later (Extended Data Fig.2g,h)....
× 106mouse CD8+T cells, 7 days after activation, were used.n = 3.h, Representative K-glutarylation western blot of immunoprecipitated PDHc as described in Fig.4g.n = 3.i, Schematic of the reactions catalysed by the individual subunits of the PDHc (adapted fromBioRender.com...
michiel1982The screenshot makes the troubleshooting much easier. I guess you want to find the lowest value in column K if column H matches your desired product "Paper". Because you use a range in IF statement instead of a single cell, you will have to use CTRL + SHIFT + ENTER after ty...
'Overflow occurs if you put more than 32,767 characters in a cell. This block allows up to 165 characters (plus delimiter) in next pass through loop. If Len(s) > 32600 Then If (i < n) And (vData(i + 1, 1) = "") Then vResults(k, 2) = s vResults(k + 1, ...
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block nonspecific binding, the tissue sections were pre-incubated with 10% normal goat serum for 30 min. Subsequently, they were incubated overnight at 4 °C with primary antibodies in a humidified chamber. The primary antibodies used to validate mast cells included mouse anti-humanCMA1(AbCam, ...
The cells were synchronized via thymidine double block (TDB) and released for the indicated times. The percent values of cells in the G1 phase are shown at right. (b) The cell cycle phase analysis of sh-NC- and sh-p38IP-transfected U937 cells exposed to phorbol myristate acetate (PMA) ...
Alternatively, the agar blocks were transferred to a tissue processor and set in a paraffin block. 10 μm slices were sectioned onto SuperFrost slides (Thermo Scientific, UK) using a microtome. After drying at 37 °C, slides were dewaxed in xylene and rehydrated in an ethanol series ...
The common gene block sequence used is previously published (Benci et al., 2019). For human cell lines, cells were stably transduced with Cas9 using the lentiviral EFS-Cas9-P2A-Puro expression vector (Addgene #108100) (Tarumoto et al., 2018). sgRNAs targeting human RIPK1 (hRIPK1) or...
Inflammasome activation leads to Caspase-1-dependent mitochondrial damage and block of mitophagy. Proc. Natl Acad. Sci. USA 111, 15514–15519 (2014). Article CAS PubMed PubMed Central Google Scholar Fouquerel, E. et al. ARTD1/PARP1 negatively regulates glycolysis by inhibiting hexokinase 1 ...