You are here: Homepage Technologies Cell-based assays Cell-based Assays IBR Inc. has a longstanding experience in development, validation and operating cell-based assays. Our special expertise are bioassays using primary cells. These cells are isolated from a broad variety of human or mammalian ...
Can be used for target validation and drug discovery, and to characterize variants of unknown significance. The complexity of ion channels and their potential cytotoxicity make developing stable cell lines particularly challenging. MaxCyte electroporated, transiently transfected Assay Ready Cells are a pro...
We demonstrated that PK parameters could be obtained by a mouse NPC binding and uptake assay using FCM, and that the obtained values were consistent with the in vivo values. We used an anti-mouse FcγRIIB antibody (2.4G2) as a model antibody for validation in mice, although the 2.4G2 ...
FDA Immunogenicity Guidance, Gene Therapy, Critical Reagents, Biomarkers and Flow Cytometry Validation (Part 3 - Recommendations on 2019 FDA Immunogenicity Guidance, Gene Therapy Bioanalytical Challenges, Strategies for Critical Reagent Management, Biomarker Assay Validation, Flow Cytometry Validation & CLSI ...
Here, we report the development of a cell-based assay based on the SmoIP sensor for the direct, quantitative, high throughput assessment of the Smo activation state. Using this system we could first demonstrate that individual cells within a largely homogenous, clonal population activate Smo at ...
or monitoring organelle function. Regardless of the type of cell-based assay being used, it is important to know how many viable cells are remaining at the end of the experiment. There are a variety of assay methods that can be used to estimate the number of viable eukaryotic cells. This ...
Training and fivefold cross-validation were repeated 100 times. Average AUC and p values are reported in Additional file 2. Prioritization of genes with the highest predictive value for classification To rank genes based on their predictive value, randomized elastic net was performed. Randomized ...
Stability-based validation of clustering solutions. Neural Comput. 2004;16:1299–323. Article PubMed Google Scholar Lord E, Willems M, Lapointe F-J, Makarenkov V. Using the stability of objects to determine the number of clusters in datasets. Inf Sci. 2017;393:29–46. Article Google ...
An apparent reluctance to explore and adopt alternative cell- and tissue-based model systems, coupled with a detachment from clinical practice during assay validation, contributes to ineffective translational research. To help address these issues and stimulate debate, here we propose a set of ...
This assay adapts a conventional ELISA format with immobilized RalGDS-RBD as a bait to selectively capture GTP-bound active Rap1. As a result, it fills an urgent need for a cell-based EPAC assay that can be conveniently performed using microtiter plates for the discovery and/or validation of...