A spectrophotometric assay for ormustine active ingredient for a liposomal dosage form with antitumor activity was selected and validated for specificity, linearity, accuracy, precision, and intermediate (intralaboratory) precision in order to ensure accurate and precise results. The obtained statistical ...
Results: A novel integration of an antiviral assay against the cytopathic effect of the encephalomyocarditis virus in HeLa cells with a very sensitive method for assay processing - the Vialight (R) Plus assay - is presented for IFN-alpha 5 activity quantification. The bioassay has been validated...
Liquid biopsy is a valuable precision oncology tool that is increasingly used as a non-invasive approach to identify biomarkers, detect resistance mutations, monitor disease burden, and identify early recurrence. The Tempus xF liquid biopsy assay is a 10
Given the importance of mouse knockout and tumor models in the development of anticancer drugs like cintirorgon, we developed our assay for various mouse matrices. To support preclinical pharmacokinetic (PK) studies in mice, an LC-MS/MS method was developed for its quantification in mouse plasma...
During early clinical development, prospective identification of a predictive biomarker and validation of an assay method may not always be feasible. Dichotomizing a continuous biomarker measure to classify responders also leads to challenges. We present a case study of a prospective–retrospective approa...
analyzers present in most hospitals and clinical laboratories. Thee4Risk testversatility, low cost, and easiness provides an excellent solution forAPOEε4 carriers identification using the same blood sample drawn for biochemical diagnostic work-up of AD patients, which can have important advantages for...
The aim of this study was thus to design and validate a grapevine STR protocol with a practically useful level of multiplexing. Using free bioinformatics tools, published primer sequences, and nucleotide databases, we constructed and optimized a primer set for the simultaneous analysis of six STR ...
This paper reports the results of a field study to validate the use of two sexing methods: (1) visual discrimination of sex vs (2) molecular sexing based on a multiplex PCR assay which amplifies the male-specific SRY gene and differentiates ZFX and ZFY gametologues. Skin samples from 628 ...
This ELISA displayed a limit of detection of 3 μg kg1of BPA in the whole product of canned vegetables and 15 μg L1of BPA in the liquid portion. For assay validation, processed vegetables were fortified with BPA at 10, 50, and 200 μg kg1. Sample treatment rendered crude and ...
We have developed an ELISA for the quantification of type III collagen degradation mediated by MMP-9 in urine. Design and methods A monoclonal antibody targeting a specific MMP-9 generated fragment of collagen III was used in a competitive ELISA. The assay was validated in urine and arterial ...