Cell-based assay methodsCells were stored on the beads, followed by a method for the cells cryogenic storage utilized in cell assays is disclosed. .FIELD 1トーマス,ニコラスケンリック,マイケルケネスムラトジック,シャロンジョアン
Cell-BasedAssay(细胞活性、毒性和凋亡)
检测原理 与流程:MSD独特的易于吸附细胞的石墨电极表面,允许悬浮细胞直接固定,无需添加其他成分。 检测结果:2-3个线性检测范围,2-3小时的简单操作流程。 案例:基因泰克应用MSDCell BasedAssay检测 anti-CD-20抗体结合活性 A high throughput electrochemiluminescent cell-binding assay for therapeutic anti-CD20 antibod...
Frozen storage of pre-transduced cells generates assay-ready cells Running assays in pharmacologically relevant cell types GeneBLAzer™ Reporter Gene AssaysThe GeneBLAzer technology utilizes a fluorescence resonance energy transfer (FRET)-based, ratiometric detection ...
Frozen storage of pre-transduced cells generates assay-ready cells Running assays in pharmacologically relevant cell types GeneBLAzer™ Reporter Gene Assays The GeneBLAzer technology utilizes a fluorescence resonance energy transfer (FRET)-...
Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5'-triphosphate in Chinese hamst... H Yin,N Pattrick,X Zhang,... - 《Analytica...
welldevelopedandcharacterizedmethod. EurofnsLancasterLaboratorieso ersextensiveex- perienceinallaspectso bioassaydevelopment,vali- dation,methodtrans erandlong-termassaymainte- nance.Weprovideadedicatedturnkeyservice orall yourbioassayneeds.Ourunmatchedexpertiseand proventrackrecord orcGMPcell-basedpotencyas- say...
We develop an assay platform utilizing donor-specific endothelial progenitor cells as a proxy to test individual sensitivity to ionizing radiation (IR). IR is known to cause long-term complications. In our method, individual IR sensitivity is correlated to an in vitro response to radiation of endo...
To extend this FCM-based method to estimating the Km and Vmax in monkey cells, an in vitro assay using monkey NPC was performed. As shown in Fig. 5a, six cell clusters (P1–P6) were identified by staining with the CD31 and CD45 marker and the expression of FcγRIIB was analyzed for...
To evaluate the ability of our γδ TCRs in activating T cells after antigen recognition, we established a cell-based assay. We generated two Jurkat-76 cell lines35that stably expressed Vγ9Vδ2 and Vγ5Vδ1 TCRs, respectively. These cells were co-cultured with K562 cells expressing BTN2...