We offer a robust suite of cell assays for our range of cell counters, making precise cell analysis easy. From apoptosis to cell vitality, our assays provide
Interestingly, both, viral particles and recombinant Spike chimeric proteins containing either the full length protein or specific domains, i.e., S1 or RBD, have been used to explore the interaction with cellular receptors in cell-based assays, as well as in vitro39,40. Here, we have carried...
based assays and how they are selected and used. Finally, we consider the in vivo relevance of in vitrocell assaysto medicinal chemistry and drug development by considering several practical examples in ion channels, G protein-coupled receptors (GPCRs), tyrosine kinase-linked receptors, anddrug ...
There are different types of bioassays to quantitatively measure biological properties, such as animal-based, cell-culture-based, and biochemical assays.3Even though animal-based assays are often most relevant to clinical response, cell-based bioassays are regarded as the most suitable for potency det...
(A) Dose-response curves of MCF 10A cells treated with eight drugs measured independently by the five centers (circles represent data from image-based assays and triangles from CellTiter-Glo assays). SeeFigure S6for underlying replicates. Dotted black lines show the dose-response curve when all ...
Mycoplasma testing (monthly) using, for example, PCR‐based kits, DNA fluorochrome staining (Figure 5), autoradiography, ELISA, immunofluorescence, biochemical assays, Routine microscopic culture observations for microbial and yeast detection, Use of routine antibiotics should be avoided, and using antibi...
Therefore, when drug candidates are being tested using cell-based assays, the methods of cell culture utilized should imitate the most natural and possible in vivo environment. Thus, 3D-cell culture is the most natural tissue-mimicking method for drug discovery. In recent years several principles ...
To enable live-cell imaging experiments, many reagents are designed for time-lapse over several hours or days, while others are optimal for end-point assays in which cells are imaged and analyzed immediately after staining. Staining conce...
Unlike traditional proliferation assays based on BrdU incorporation, no DNA heat- or acid-denaturation steps are required with SBIP in order to visualize the labeled strand breaks, allowing simultaneous detection of the morphology ...
To verify this, we performed Transwell cell migration assays on X0 and XY cell lines and the obtained data were quantified through crystal violet staining and measured at 570 nm (Fig. 7A). As shown in the plot, the migratory ability of Y-depleted cells was significantly enhanced and well ...