The viral receptor ACE2 mRNA expression was specifically down-regulated in SARS-CoV-2-infected Caco-2 cells, while it remained stable in HCoV-OC43-infected Caco-2 cells, a virus which uses HLA class I instead of ACE2 to enter cells. It is worth noting that SARS-CoV-2 induces lower ...
Knock down of DNMT1 expression by siRNA induced the promoter of CDH1 demethylation and upregulated CDH1 transcription. High-density oligonucleotide gene expression microarrays were used to examine the effects of DNMT1 knock down on human HCC cells (SMMC-7721); these showed that a number of genes...
摘要: Review on CDH1 (cadherin 1, type 1, E-cadherin (epithelial)), with data on DNA, on the protein encoded, and where the gene is implicated.关键词:Chromosome 16 CDH1 gene Arc-1 (alias CD324 (alias CDHE (alias Cadherin-1 (alias E-cadherin (alias ECAD (alias LCAM (alias UVO (...
GeneID83502 Gene SymbolCDH1 Directions for useResuspend lyophilized shRNA plasmid DNA in 500 μl of deionised water. Each vial contains 50 μg of lyophilized shRNA plasmid DNA. Suitable for up to 50 transfections. Quality ControlThe sequence of shRNA is guaranteed by sequencing. ...
pCI-HA-NEDD4 (27002) and pCI-HA-NEDD4L (27000) [46] were purchased from Addgene (Cambridge, MA, USA) and were subcloned into pCMV-Flag to generate Flag-NEDD4 and Flag-NEDD4L, respectively. His-Ubiquitin, HA-Cdh1, HA-Cdc20, Myc-Cdh1, pBabe-HA-Cdh1, pGEX-Cdh1, Flag-Smurf1 and...
Droufakou S, Deshmane V, Roylance R, Hanby A, Tomlinson I, Hart IR (2001) Multiple ways of silencing E-cadherin gene expression in lobular carcinoma of the breast. Int J Cancer 92(3): 404–408. Fisher B, Costantino JP, Wickerham DL, Cecchini RS, Cronin WM, Robidoux A, Bevers TB...
MiR-217 can function as an oncogene or a tumour suppressor gene depending on cell type. However, the function of miR-217 in lung cancer remains unclear to ... Junhua,Guo,Zhijun,... - 《Molecules & Cells》 被引量: 42发表: 2014年 The microRNA-217 functions as a tumor suppressor and ...
Immune infiltration levels were evaluated by single-sample GSEA (ssGSEA) using the “GSVA” R package, which can determine the immune cell population in a tumor sample according to gene expression data [28]. The infiltration enrichment of 24 common immune cell types was computed, including B ce...
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② Rotor-Gene 3000Realtime PCR 仪上进行PCR 反应。反应条件:94℃预变性10s, 94℃变性5s,60℃退火及延伸20s,共反应40 个循环。 ③分析融链曲线,以△Ct 表示 Cdh1 的表达,△Ct=Ct(Cdh1)- Ct(β-actin ), △Ct 值越高,Cdh1 的表达越低 [16] ...