The frequency of bright double-positive CD45RA[sup +],CD45RO[sup +] T cells, which represent cells in transition from the CD45RA[sup +] to CD45RO[sup +] phenotype, was higher in the adenoids than in peripheral blood. The frequency of another double-positive cell population, but with...
Separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD45RA (MEM-56) PE Monoclonal antibody (Product # MA1-19636) using a dilution of 20 µL re...
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hemato...
of 220 kDa. B220 * is not restricted to B cells and can also be expressed on activated T cells, on a subset of dendritic cells and other antigen presenting cells. Naive T lymphocytes express large CD45 isoforms and are usually positive for CD45RA. Activated and memory T lymphocytes ...
It is now recognized that naive T lymphocytes express the high molecular weight isoform containing the A exon (CD45RA) and that is lost after activation and replaced by the low molecular weight isoform CD45RO [5]. Therefore CD45RA expressing T cells were initially considered to be naïve ...
CD45RA is typically found on naïve T cells that are mature but have not yet been activated in the periphery. CD45RO is most commonly expressed on the surface of memory T cells, as well as other lymphocytes such as B cells. CD45RO facilitates signaling-mediated activation of T. ...
mAb 5A2.G5 displayed weaker binding to all lymphoid cell types, indicating that the pattern of glycosylation of the CD31 antigen differs between lymphocytes (of all types) and cells of the myeloid lineages. The heterogeneity of CD31 antigen expression by CD4-positive cells was further examined ...
Intraepithelial T cells of the TcR gamma/delta+ CD8- and V delta 1/J delta 1+ phenotypes are increased in coeliac disease. Expression of the gamma/delta T-cell receptor (TcR) for antigen on CD3+ intraepithelial lymphocytes (IEL) was studied in situ by two-colour immunofluorescence on ...
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hemato...
(B) Composition of the different T-cell subsets of CD8+ CMV-specific T cells was determined based on 4-color immunostaining of CD27-FITC, CD45RA-PE, CD8-PercP, and CMV-tetramer-APC. Cells were gated on lymphocytes via FSC/SSC, and CMV-specific CD8+ T cells were additionally gated on...