A cell expressing CD40L ligand (CD40L) for B cell activation is provided to obtain an activated B cell whose antigen presentation and proliferation are improved and develop a drug of antivirus or anti-tumor. A cell expressing CD40L for enhancing B cell proliferation and antigen presentation is ...
for helpful comments. The CD40-293-SEAP cell line was kindly provided by Richard Kornbluth (Multimeric Biotherapeutics, Inc.). Human gp100 cDNA was kindly provided by Patrick Hwu (MD Anderson Cancer Center). GVAX (B16-F10 cell line expressing murine GM-CSF) was kindly provided ...
To discriminate between the role of CD40L on APCs and that of CD8+ T cells, we generated mixed BM chimeras that would allow us to examine independently the different cell types expressing CD40L. Irradiated mice were reconstituted with equal parts of bm1 and Cd40lg−/− BM. Bm1 mice ...
TMZ-CD40L-expressing tumor cells promote M1 macrophages As macrophage stimulation seems crucial for anti-tumor responses in pancreatic cancer,4 the pancreatic cancer cell line MiaPaCa2 was co-cultured with human monocytes after being infected with the LOAd viruses or left uninfected. Co-culture of ...
The consequences of Tim-3 signaling on T cells have been the focus of numerous studies;40 however, recent reports established that there is a reciprocal signal transmitted to the participating Gal-9-expressing antigen presenting cell. Jayaraman et al.22 demonstrated that Tim-3 expressed on Th1 ...
However, as also reported in specific human and mice mucosal compartments (Arpin et al., 1998; Chen et al., 2009; Koelsch et al., 2007), B cells exclusively expressing IgD on the cell surface have been identified in catfish (Ictalurus punctatus) blood (Edholm et al., 2010) or rainbow...
In an embodiment of the present invention, the interaction may occur at the cell surface and a method for blocking the cell surface interaction between CD40L and α5β1 integrin may comprise contacting cells (a cell expressing CD40L and/or a cell expressing α5β1 integrin) with an effective...
GM.CD40L.CCL21 (Arm B) in patients with lung adenocarcinoma who had failed first-line therapy. Primary endpoints were safety and tolerability of Arm B in phase I and progression-free survival (PFS) in phase II; secondary endpoints included anti-tumor immune responses/T...
( 2007 ) A phase-I trial using a universal GM-CSF-producing and CD40L-expressing bystander cell line (GM.CD40L) in the formulation of autologous tumor cell-based vaccines for cancer patients with stage IV disease . Annals of Surgical Oncology , 14 , 869 – 884 ....
(SEQ ID NO:5).A CHO cell line constitutively expressing the entire human Mac-1 α-chain served as template. The resulting PCR-fragment was cloned in pGEMT (Promega), released byNcoI andNotI (New England Biolabs) and inserted into the Ncol-Notl-linearized pET20b. This expression vector was...