Resting CD4+ T-cell populations from human immunodeficiency virus type 1 (HIV-1)-infected individuals include cells with integrated HIV-1 DNA. In individuals showing suppression of viremia during highly active antiretroviral therapy (HAART), resting CD4+ T-cell populations do not produce virus ...
CD4+ T细胞指的是表面有CD4+ T分子的T淋巴细胞，是人体免疫系统中的一种重要免疫细胞，由于艾滋病病毒攻击对象是CD4+ T细胞，所以其检测结果对艾滋病治疗效果的判断和对患者免疫功能的判断有重要作用。全球仍有约3700万人感染了HIV病毒。这套方案克服了CD4+ T细胞对基因操作的抵抗力，并与改进后的培养条件相结合，...
近日,南方科技大学靳文菲课题组在iScience上发表了题为“Exploring the dynamics and influencing factors of CD4 T cell activation using single-cell RNA-seq”的研究。该研究利用抗CD3/CD28磁珠刺激人静息态T细胞,并对刺激前后的样本进行单细胞转录组测序(scRNA-seq),整合公共数据从细胞亚群、基因表达、细胞-细胞通讯...
近日,这项研究的研究结果发表在《Nature Methods》上,题为“Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4 T cells allows complex functional analyses+”。 https://doi.org/10.1038/s4...
1 infection can persist despite ART in the resting CD4⁺ T cell reservoir of hu-Rag2⁻/⁻ γ(c)⁻/⁻ mice. This model will allow rapid preliminary assessments of novel eradication approaches and combinatorial strategies that may be challenging to perform in the NHP model or in humans...
CD4 T cells are the major targets of HIV-1 in vivo, and their permissivity to productive infection is dictated by their activation state. The CD4 T cell repertoire residing within lymphoid organs and circulating in peripheral blood is largely resting without cell proliferation and lacks expression...
HIV type 1 (HIV-1) persists within resting CD4 T cells despite antiretroviral therapy (ART). To better understand the kinetics by which resting cell infect... Nancie M.Archin,Naveen K.Vaidya,JoAnn D.Kuruc,... - 《Proceedings of the National Academy of Sciences of the United States of Am...
This proposed immune evasion mechanism likely also exists in resting CD4+ T-cells. In addition, HIV-1 restriction mediated by SAMHD1 in resting CD4+ T-cells may contribute to viral immunopathogenesis, such as T-cell depletion and establishment of the viral reservoir and latency (Figure 1). ...
Efficient delivery of lentiviral vectors into resting human CD4 T cells. Gene therapy. 2014;21(4):444-9.Geng X, Doitsh G, Yang Z, Galloway NL, ... X Geng,G Doitsh,Z Yang,... - 《Gene Therapy》 被引量: 0发表: 2016年 Efficient delivery of lentiviral vectors into resting human CD4...
CD4+ T cells help antiviral CD8+ T cells in two main ways: they maximize CD8+ T cell population expansion during a primary immune response and also facilitate the generation of virus-specific memory CD8+ T cell populations. In addition to their helper functions, CD4+ T cells contribute direct...