Resting regulatory CD4 T cells: a site of HIV persistence in patients on long-term effective antiretroviral therapy. PLoS One. 2008;3, e3305.Tran, T. A., M. G. de Goer de Herve, H. Hendel-Chavez, B. Dembele, E. Le Nevot, K. Abbed, C. Pallier, C. Goujard, J. Gasnault, J...
Methods Here, using magnetic beads and fluorescence- activated cell sorting, we extensively phenotype the Tck effector cells and conclude that effector function resides within the CD4+CD45RO+, CCR7-, CD49dhigh population, and that these cells are derived from the effector memory CD4+ T cells ...
Replication-competent virus was recovered from pooled resting CD4 T cells in 7 of 16 mice, with a median frequency of 8 (range, 2 to 12) infected cells per million T cells, demonstrating that HIV-1 infection can persist despite ART in the resting CD4 T cell reservoir of hu-Rag2/ γ(...
The hallmark of HIV-1 disease is the gradual disappearance of CD4+ T cells from the blood. The mechanism of this depletion, however, is still unclear. Evidence suggests that lymphocytes die in lymph nodes, not in blood, and that uninfected bystander cells are the predominant cells dying. Our...
These results suggest that the intracellular dNTP pools in resting CD4+ T-cells are rate limiting for HIV-1 reverse transcription, and SAMHD1 plays a regulatory role in this process. Furthermore, silencing SAMHD1 expression in post-activated resting CD4+ T-cells using specific siRNA or shRNA ...
Altered regulatory T cell homeostasis in patients with CD4+ lymphopenia following allogeneic hematopoietic stem cell transplantation CD4+CD25+Foxp3+ Tregs have an indispensable role in the maintenance of tolerance after allogeneic HSC transplantation (HSCT). Patients with chronic graft-v... KI Matsuoka,...
These results suggest that the intracellular dNTP pools in resting CD4+ T-cells are rate limiting for HIV-1 reverse transcription, and SAMHD1 plays a regulatory role in this process. Furthermore, silencing SAMHD1 expression in post-activated resting CD4+ T-cells using specific siRNA or shRNA ...
Resting CD4+ T cells isolated from PI- treated patients compared to NNRTI-treated patients showed a limited HIV-1 reactivation upon T-cell stimulation (p = 0·024) and a lower level of HIV-1 integration (p = 0·024). Our study indicates that PI- based cART could be more ...
Monocytes are able to induce latency in resting CD4+T-cells We have previously reported that mDC, but not pDC, are able to efficiently induce post-integration latent infection in resting CD4+T-cells using an in vitro DC-T-cell co-culture model [14]. However, mDC and their subpopulations ...
CD4(+) T cell and NK cell interplay key to regression of MHC class I(low) tumors upon TLR7/8 agonist therapy. One of the next challenges in cancer immunotherapy is the resistance of tumors to T-cell–based treatments through loss of MHC class I. Here, we show that under these circumsta...