北京泽平代理的美国进口LONZA人骨髓造血干细胞(Human Bone Marrow CD34+ Stem/Progenitor Cells,或Human Bone Marrow Derived Hematopoietic Stem/Progenitor Cells),具备庞大的供体库,为复杂研究提供了更多便利性,不论是需要单一供体大量细胞的研究,还是对不同年龄、性别、种族供体细胞间差异的研究,均可获得理想的细胞产品。
北京泽平代理的LONZA人脐带血CD34+造血干细胞及培养基,供体来源丰富,本款冷冻保存的人脐带血CD34+造血干细胞,由单个健康供体的脐带血单核细胞(MNCs)通过阳性免疫磁性分离得到,并通过CD34+表达验证,可保证纯度≥90%。其所有细胞均不含有HIV-1和乙型肝炎,每个批次细胞均可提供质量分析报告。每支含有≥1,000,000个细...
Bone marrow CD34+ cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types. Purity and Viability You Can Count On Lonza bone marrow CD34+ cells are isolated from bone marrow mononuclear cells (MNC) by positive immunomagnetic separatio...
Human CD34 Cell Nucleofector® Kit (Lonza, Koln, Germany) was used. Nucleofection was performed with Amaxa Nucleofector II using program U-008. Immediately after nucleo- fection, cells were suspended in HSC medium and gently trans- ferred to a CH-296-pretreated non-TC well plate and spun ...
英文名称:Human Cord Blood CD34+ Stem/Progenitor Cells, Cryopreserved, 1 Million LONZA脐血CD34+细胞结合配套的LONZA细胞生长培养基,进行无血清培养时,细胞性能表现更佳: 培养基货号:PT-3926 中文名称:HPGM造血细胞无血清培养基 英文名称:HPGM Hematopoietic Progenitor Growth Medium ...
The plasmid pmaxGFP (Lonza) was used in experiments described in Figure 5 as a means to transiently label transfected cells. Sources of Primary Cells Frozen primary CD34+ cells purified from umbilical cord blood or adult bone marrow were obtained from AllCells (for cord blood, products CB005F...
G-CSF-mobilized peripheral blood CD34+ cells (LONZA, Walkersville, MD, USA) were transduced with the MSCV-c-KIT(N822K) vector or mock-transduced and eGFP+ cells were FACS sorted 6 days after transduction. Stably transduced U937 cells were FACS sorted before analysis. Total RNA (input: 40...
Cells 4 Additional Information For an up-to-date list o all Nucleo ector® Re erences, please re er to: .lonza/nucleofection-citations For more technical assistance, contact our Scientific Support Team: USA /Canada Europe and Rest o World ...
Transfections were performed using the Nucleofector system and the Amaxa Cell Line Nucleofactor Kit V (Lonza, Verviers, Belgium). The best efficiency of HtrA2/Omi small interfering RNA was determined at 48 hours and 400 nM. Transfection efficiency was validated by Western blot by using anti-...
(CB) CD34+ cells. Methods Two sets of non-integrating plasmids were respectively transported into two groups of short-term cultured CB CD34+ cells by using transfector nucles, to reprogram iPSCs from CB CD34+ cells. Within 14 days of transfection by two sets of non-integrating plasmids, up...