Median number of bone marrow nucleated cells and CD34(+) cells infused were 2.4 x 10(8)/kg and 3.7 x 10(6)/kg, respectively. A CD34(+) cell dose of 3 x 10(6)/kg or more significantly influenced neutrophil (hazard ratio [HR] = 1.37, P =.04), monocyte (HR = 1.47, P =.02...
(A)和(B) 分别用美天旎Monocyte Isolation Kit II human和无柱式分选系统阴性分离。
The Cd34-lineage tdT+ cells were wildly distributed in mesenchymal cells, ECs, and monocyte/macrophage populations. Previous studies confirmed the role of bone marrow-originated Cd34-derived myeloid cells in cardiovascular diseases, which is a distinct developmental origin from their tissue-resident ...
Methods for enriching CD34 + human hematopoietic progenitor cells The present invention relates to methods of enriching hematopoietic progenitor cells from body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a gradient solution adjusted to a specific density ...
32 Briefly, 105 allogeneic monocyte-depleted mononuclear cells were incubated in round bottom 96-well tissue culture plates with graded doses of stimulators (30,000 to 3,300) irradiated at 3,000 rads. After 5 days, cultures were pulsed with 1 μCi/well of 3H-thymidine (New England Nuclear...
20 More recently, the role of CD155 (PVR), a member of the nectin family, and its ligand DNAM-1, in monocyte transmigration through human umbilical vein endothelial cells (HUVECs) has been evidenced, using either blocking monoclonal antibodies (mAbs) or soluble forms of these molecules.21,...
Cells were stained for CD34ϩ using the International Society of Hematotherapy and Graft Engineering (ISHAGE) definition for identifying CD34ϩ, i.e. cells gated on CD45ϩ dim/low side scatter CD34ϩ positive cells (17). Samples were acquired using a FACS Calibur flow cytometer (BD ...
In some instances, two or three CML patient samples were pooled after thawing in order to obtain sufficient numbers of CD34 þ cells for the present studies. Isolation of HSC/PC sub-populations by multicolor flow cytometry Enriched CD34 þ cells were stained simultaneously with FITC–CD34, ...
Natural killer (NK) cells were defined as MNCs expressing CD16 or CD56 and not expressing CD3 or CD14. Absolute counts (per unit volume) were calculated as percentages (determined by flow cytometry) multiplied by absolute lymphocyte plus monocyte counts (determined by the clinical hematology ...
Mesenchymal stem cells inhibit generation and function of both CD34+ -derived and monocyte-derived dendritic cells. J. Immunol. 177: 2080-2087.Nauta AJ, Kruisselbrink AB, Lurvink E, Willemze R, Fibbe WE. Mes- enchymal stem cells inhibit generation and function of both CD341- derived and ...