Based on phenotype (surface marker profile and receptor expression) and function, both mouse and human monocytes can be divided into two main subsets: classical monocytes (Gr1+/CD43−/CD62L+/CX3CR1low/CCR2+/VEGFR1high cells in the mouse and CD14high/CD16−/CD62L+/CX3CR1low/CCR2+/...
In the final MDM-QVOA assay, TCRβ was used to estimate the absolute number of CD3+ T cells in MDM-QVOA (see Extended Data Table 4 for examples of how we calculated the number of CD3+ cells in the T cell control wells). In brief, we assessed the number of TCRβ RNA copies and...
Absolute counts (cells/L) of total monocytes by classical, intermediate, transitional and non-classical monocyte subset components by HOMA-IR quartiles.doi:10.1371/journal.pone.0090330.g003M. CeciliaC. DominicM. LouieZhang GuangxiangM...
The same holds true when stratifying the patients by relative (percentage of CD14++CD16+ cells among all monocytes) rather than absolute (cells μl−1) cell counts (data not shown). In contrast, neither total monocyte counts nor counts of CD14++CD16− monocytes or CD14+CD16+ monocytes ...
Indeed, we observed an absolute predominance of binucleated cells in cMoP compared to MC after exposure to BCG (Supplementary Fig. 1c). Yet, the total number of cells with two nuclei was highest in cMoP treated with M-CSF alone (Supplementary Fig. 1d). This was likely due to the ...
The absolute lymphocyte/monocyte ratio recovery during ABVD treatment cycles is not significantly impacted by the use of myeloid growth factors and predict... Risk stratification of patients with classical Hodgkin lymphoma (cHL) remains suboptimal. The ratio of the absolute lymphocyte count (ALC) to ...
In the brain, infiltrating Mo/MΦs were identified as CD45+/CD11b+/Ly6G−/CX3CR1+/Ly6C+ [36]. The absolute count of cells was determined by flow cytometry using Counting Beads (424902, Biolegend). Ly6C-positive cells were enriched after the isolation of single cells from the blood ...
Also, the absolute monocyte count, as well as all other leukocyte populations, in lean and obese individuals, increased after exercise. This fact may partially account for the observed reduction in the percentage of CD16+ cells in response to exercise. Insulin-resistant, but not insulin-sensitive...
CD45 gate was set where monocytes typically reside. Other lineages were excluded using antibody combinations in the panel; T cells were excluded with CD3, B-cells and granulocytes with CD24, NK cells with CD7 and CD56. The purified monocytes were then compartmentalized into the MO1, MO2, ...
Platelets and white blood cells (WBC) were enumerated using an automatic cell counter (HEMAVET; Drew Scientific). Absolute monocyte counts were calculated from total WBC counts and the proportion of CD115+ cells out of total CD45+ cells identified by flow cytometry. Gadolinium chloride (GdCl3)....