CD11c DTR mice were also used to investigate the role of CD11c cells, M1 M and DCs in folliculogenesis. In WT mice, the proportion of CD206+ M2-like Ms was not increased in follicular induction, while that of CD11c+ M1-like Ms was increased. In CD206 DTR mice, folliculogenesis was ...
Diphtheria toxin depletes CD11c-DTR mice of CD11c+ cells.Frida HenningssonZhoujie DingJoakim S. DahlinMarius LinkeviciusFredrik CarlssonKjellOlov GrönvikJenny HallgrenBirgitta Heyman
Significantly, CD11chiPDCA-1int/lo DC-depleted mice had early recruitment of inflammatory cells into the brain, compared to vehicle-treated CD11c-DTR mice after JEV infection (Fig. 2A–F). The majority of infected cells were observed to be neuron cells, since prominent costain- ing was ...
(B) Total splenocytes from WT or CD11c-DTR mice were cocultured with MSCs at a 10:1 ratio (splenocyte : MSC) with low-dose recombinant IL-2 (10U/ml) for 72 h and the CD4+FoxP3+ cell population was analyzed by flow cytometry. doi: 10.1371/journal.pone.0072544...
取DTR/GFP小鼠,注射白喉毒素(16ng/g体质量)18h后,建立IR模型,于再灌注24h后,检测肾功能变化、肾组织病理改变、rIX2比例及血清中的白细胞介素10(IL-10)、肿瘤坏死因子-α(TNF-α)、MCP-1等细胞因子变化。结果小鼠注射16ng/g体质量白喉毒素后可有效清除rDC并维持小鼠最小损伤。清除rDC后小鼠肾功能损害明显加重...
and the expression of serum TNF-α IL-10,IL-6 and MCP-1 was measured with ELISA kit.Result The intraperitoneal injection of 16 ng/g weight DT displayed highest clearance of renal resident DCs and minimal weight loss in DTR/GFP mice.In the absence of CD11c+ F4/80 renal resid...
Lung TNF-α levels were drastically reduced in CD11c-DTR BALB/c mice depleted of CD11c+ cells, and TNF-α-producing Ly6c(+)CD11b(+) cells were ... M Fei,S Bhatia,TB Oriss,... - 《Proceedings of the National Academy of Sciences》 被引量: 166发表: 2011年 Resident CD11b+Ly6C Lung...
Methods: CD11c‐DTR+ and CD11c‐DTR‐ mice (5 months, both sexes) were used. Both groups received single or repetitive (every 2 days for 8 days) subconjunctival injections of DT or its vehicle (PBS). Basal tearing (in mm) was measured using phenol red threads placed in the temporally ...
However, CD11c.LuciDTR and CD11c.DTR mice showed late neutrophilia 72 h after dendritic cell depletion, which was independent of PMN release and possibly resulted from increased granulopoiesis. Thus, the time point of dendritic cell depletion and the choice of DTR transgenic mouse line must be ...
However all these effects induced by the MSCs were partially abrogated when CD11c+ cells were depleted in the CD11c+-DTR transgenic mice. In addition, the observation that adoptive transfer of WT CD11c+ cells partially restored the beneficial effect of the MSCs, while transferring IL-10 deficient...