The aim of this work was to validate the CD11c‐DTR+ mouse model proving that the observed effects are not due to the DT itself, using littermates negative for the transgene and therefore resistant to DC depletion by DT. Methods: CD11c‐DTR+ and CD11c‐DTR‐ mice (5 months, both sexes...
CD206 or CD11c diphtheria toxin receptor transgenic (DTR) mice, which enable depletion of CD206+ M2 Ms and CD11c+ M or CD11c+ Dendritic cells (DCs), respectively, were used. Oocytes were used for in vitro fertilization and embryo transfer. In vitro fertilized embryos derived from M2 M ...
In addition, we also found that increased FoxP3 mRNA expression in kidneys of MSC treated IR mice was partially suppressed when CD11c+cells were predepleted using CD11c DTR transgenic mice, suggesting the important contribution of CD11c+ cells as well as Tregs in the renoprotecti...
Results Selective ablation of CD11chiPDCA-1int/lo DCs, but not CD11cintPDCA-1hi pDCs, exacerbates JE. CD11c-DTR transgenic mice, which express diphtheria toxin receptor (DTR) gene under con- trol of a cloned Itgax promoter and thus allow conditional DC depletion upon DT injection, has ...
DTR transgenic mice were injected intraperitoneally (i.p.) with diphtheria toxin (Sigma-Aldrich, St. Louis, MO) at a concentration of 9 ng/g of body weight suspended in phosphate-buffered saline (PBS), or they were given PBS alone. Prior to undertaking the experiments described herein, the...
To investigate the dependence of individual immunological processes on DC, a transgenic mouse system (CD11c-DTR/GFP mice) has been developed that allows conditional depletion of CD11c+ DC in vivo through administration of diphtheria toxin. We have performed careful histological analysis of CD11c-DTR/...
which depends only on the innate immune system, we investigated the innate role role of dendritic cells in inflammatory arthritis Methods KBxN serum transfer arthritis was induced in CD11c-diphteria toxin receptor (DTR) transgenic mice, which express the human diphtheria-toxin receptor under the CD1...
Histological analysis found that CD11c-DTR transgenic mice that had received DT displayed decreased synovial inflammation and a trend towards reduced local bone destruction.ConclusionsThese data show that dendritic cells are involved in innate reactions leading to inflammatory arthritis and suggest that ...
Methods: To assess the role if CD11c-expressing intimal myeloid cells in early atherosclerosis, CD11c + cells were deleted in Ldlr -/- mice reconstituted with bone marrow from CD11c-DTR transgenic mice. Mice reconstituted with C57BL/6 bone marrow were used as controls. Depletion of CD11c + ...
Objective We aimed to investigate the impact of depleting CD11c+ myeloid cells, including DCs and macrophages, during bleomycin-induced pulmonary fibrosis in mice. Methods We used a diphtheria toxin (DTx) receptor (DTR) transgenic mouse model (CD11c-DTR-Tg) to deplete CD11c+ myeloid cells through...