黑框圈出的细胞群为Ly6GhighG-MDSC,红框圈出的细胞群为Ly6GlowG-MDSC(图4A1和B1)。4T1和4T07对照组Ly6GlowG-MDSC亚群的百分比分别为(13.8±2.0)%和(14.2±2.7)%,而禁食组的比例分别降至(6.2±4.7)%和(4.5±3.8)%(图4A2...
Recruited CD11b+Ly6G+ immature myeloid cells promote liver regeneration following major liver resectionBackground Application of minimally invasive surgery for oncologic liver resection is still limited to expert centers. We describe our experience in laparoscopic liver resection (LLR) for colorectal liver ...
骨髓来源的抑制性细胞(Myeloid-derived suppres洋战尼行sor cells,MDSCs)是骨髓来源的一群异质性细胞,是树突状细胞(dendritic cells,DCs)、巨噬细胞和(或)粒细胞的前体,具有显著抑制免富烈绍学治盾济条免疫细胞应答的能力。 小鼠MDSCs的表型为CD11b+Gr-1+,分两个亚型:粒细胞样的MDSCs(CD11b+Ly6C-Ly6G+)和...
Ly6G - cells show distinct functionin mice with chronic inflammation or tumorburdenEva Källberg, Martin Stenström, David Liberg, Fredrik Ivars and Tomas Leanderson *AbstractBackground: S100A9 has been shown to be important for the function of so called Myeloid Derived SuppressorCells (MDSC). ...
S100A9 has been shown to be important for the function of so called Myeloid Derived Suppressor Cells (MDSC). Cells with a similar phenotype are also involved in pro-inflammatory processes, and we therefore wanted to investigate the gene expression and fu
用于检测细胞表面蛋白的抗体包括:购自ebioscience的CD3e、CD4、CD8a、CD19、NK1.1、F4/80、CD45、CD11b、CD45.1、CD45.2、Ki67、Ly6C和购自biolegend的CD115、Ly6G、和Siglec-F。IL4Rα抗体购自R&D。 通过在消化前以和胶原酶为1:1000比例加入GolgiPlug,进行脂肪巨噬细 胞胞内RELM-α染色。在表面分子染色完成...
FSC-W cells excluded doublet cells. Ly6g-phycoerythrin was then used to negatively gate out all neutrophils, and all lymphocytes were gated out based on their lower FSC-A and SSC-A profile. This allowed FACS analysis on the remaining M^/monocyte subpopulations. AMFs were discriminated from ...
Representative dot plots of three mice indicating CD11bLy6G þ and CD11bLy6C þ cells (d) and bar chart of 3–4 mice±s.d. Student's t-test significance: **P40.005. (f) Intestinal permeability as determined by quantifying the amount of fluorescein isothiocyanate (FITC)–dextran levels (...
Cells were stained with the following mAbs (BD Pharmingen): anti-CD11c (HL3, biotinylated or phycoerythrin conjugated), anti-CD11b (M1/70, allophycocyanin conjugated), or/and anti-Ly6G/C (Gr-1) (RB6-8C5, phycoerythrin or fluorescein isothiocyanate (FITC) conjugated). Allophycocyanin or peridin...
However, sorted CD11b+Gr1+ cells (comprising more than 90% of CD11b+Ly6G+Ly6Cint cells) from MPA-treated tumor bearing mice displayed a more pronounced suppressive activity on NK cell degranulation in response to YAC-1 cells and a stronger inhibition of IFN-g production of NK cells in ...