与Ly6GhighG-MDSC亚群相比,在所有66个使用的标记物中,只有CXCR4、F4/80、MHC II和CD205在Ly6GlowG-MDSC中高表达(图4C)。此外,对Ly6Glow和Ly6Ghigh的G-MDSC亚群进行分类,通过质谱检测蛋白表达差异(图4D1)。研究发现,与Ly6GlowG-M...
RESEARCH ARTICLE Open AccessCD11b + Ly6C ++ Ly6G - cells show distinct functionin mice with chronic inflammation or tumorburdenEva Källberg, Martin Stenström, David Liberg, Fredrik Ivars and Tomas Leanderson *AbstractBackground: S100A9 has been shown to be important for the function of so ...
磁珠分选脾CD4^+CD25-T细胞与纯化Gr1^+细胞共培养检测T细胞增殖能力.结果慢性束缚心理应激导致小鼠骨髓中CD11b^+Gr1^+细胞的子聚集,细胞亚群主要为Ly6G^+Ly6Clow的不成熟中性粒细胞,抑制了脂多糖(LPS)诱导下巨噬细胞产生IL-6/TNF-α,并抑制了T细胞的增殖.结论慢性束缚心理应激诱导小鼠CD11b^+Gr1^+髓系细胞的...
Arginase I and iNOS expression could be detected in both CD11b+Ly6C+Ly6G+ and CD11b+Ly6C+G-/C++G- derived from tumors or a site of chronic inflammation, but was very low in the same cell populations isolated from the spleen. CD11b+ cells isolated from mice with peritoneal chronic ...
Methods CD11b+Ly6C++ and Ly6G+ cells were isolated from spleen, tumor tissue or inflammatory granulomas. S100A9, Arginase 1 and iNOS gene expression in the various CD11b+ cell populations was analyzed using Q-PCR. The suppressive activity of the CD11b+ cell populations from different donors wa...
Cells were stained with the following mAbs (BD Pharmingen): anti-CD11c (HL3, biotinylated or phycoerythrin conjugated), anti-CD11b (M1/70, allophycocyanin conjugated), or/and anti-Ly6G/C (Gr-1) (RB6-8C5, phycoerythrin or fluorescein isothiocyanate (FITC) conjugated). Allophycocyanin or peridin...
The Ly6G+Ly6Clow subset of MDSCs was significantly larger during CD11b+ myeloid cell-mediated tumor growth. We demonstrated that Gr-1+CD11b+ MDSCs accumulated in the tumor cells in the tumor microenvironment of the ApcMin/+ mice, but the number of Gr-1+ cells decreased in the tumor ...
Representative dot plots of three mice indicating CD11bLy6G þ and CD11bLy6C þ cells (d) and bar chart of 3–4 mice±s.d. Student's t-test significance: **P40.005. (f) Intestinal permeability as determined by quantifying the amount of fluorescein isothiocyanate (FITC)–dextran levels (...
anti-mouse Ly6C (HK1.4) Fluidigm Cat# 3150010BRRID: AB_2895118 anti-mouse Ly6G (1A8) Fluidigm Cat# 3151010B anti-mouse CD64 (X54-5/7.1) Biolegend Cat# 139301RRID: AB_10612757 anti-mouse Tim4 (RMT4-54) Biolegend Cat# 130002RRID: AB_1227802 anti-mouse XCR1(Zet) Biolegend Cat# ...
The CD11b+Ly6Chigh inflamed monocytes, CD11b+Ly6Clow resident monocytes, CD11b+Ly6G+ neutrophils, CD11b+F4/80+ macrophages and CD11b+CD19+ B cells were expanded in the spleen and BM during the progression of melioidosis. After adoptive transfer of CD11b populations harboring B. pseudomallei,...