2、流式检测的细胞群体: •中性粒细胞 (Ly6G + CD11b + CD64-)•肺泡巨噬细胞 (Ly6G-CD64 + SiglecF + CD11c +)•单核细胞(CD64-Ly6C-和CD64-Ly6C +)•炎性单核细胞 (CD64 + Ly6C +)•间质巨噬细胞样细胞 (F4/80 + CD11b + Ly6...
2、流式检测的细胞群体: •中性粒细胞 (Ly6G + CD11b + CD64-)•肺泡巨噬细胞 (Ly6G-CD64 + SiglecF + CD11c +)•单核细胞(CD64-Ly6C-和CD64-Ly6C +)•炎性单核细胞 (CD64 + Ly6C +)•间质巨噬细胞样细胞 (F4/80 + CD11b + Ly6G-SiglecF-Ly6C-CD64 +)•Ly6G+ 巨噬细胞 (Ly6G...
该文献系 12 年发表,虽历时已久,然其经典价值恒在,值得当下借鉴。文中有两大创新亮点。其一,以 Ly6C/Ly6G 检测髓系分群相较 Gr-1 更具优势;其二,在检测髓系细胞时,F4/80 并非必需抗体,因其染色过程实验质量波动较大。且常用检测髓系细胞的标志物(CD11b、CD11c、F4/80、Gr-1、Ly6C 及 Ly6G)尚无标准流程...
在该模型中,IAV感染后第5天,Ly6G+CD11b+CD64−中性粒细胞(Neus)数量增加,第15天恢复到基线水平(图1,A和B)。在感染流感病毒后的第5天至第10天,观察到部分Ly6G-CD64+SiglecF+CD11c+ AMs丢失,在第15天恢复,如前所述(图1,A和B)。CD64-Ly6C-单核细胞(Ly6C-Mos)的数量在感染过程中保持稳定,而经典的CD...
(MHCII+ CD11c+ CD11b−) (n=6). At 12, 24 and 48 h after the administration of MTDs (n=3), (c) miR-223−/+ and (d) WT mouse myeloid cells were sorted, and the expression of mmu-miR-223 was detected by TaqMan. All data are presented as the mean±S.E.M., and ...
B. CD11b+ cells sorted from peritoneal exudate cells (PEC) of normal control or granuloma-carrying BALB/c mice were co-cultured with splenic CD4+ T cells and CD11c+ cells isolated form BALB/c mice and proliferation determined as in A. Dashed red lines in A and B indicate unsuppressed T...
After labelling with specific antibodies, including MHCII, CD11b, LY6C, LY6G and CD11c, flow cytometry was performed to identify neutrophils (Ly6G+ MHCII−), monocytes (Ly6C+ MHCII−), intermediate monocytes (Ly6C+ MHCII+), macrophages (Ly6C− MHCII+) and dendritic cells (MHCII+ CD11c...
Neutrophils express the β2 integrins LFA-1 and Mac-1, and, in lower abundance, complement receptor 4 (CD11c/CD18). The role of LFA-1 and Mac-1 in migration has been studied in many disease models. Where published results diverge, it can be difficult to differentiate organ and/or ...
•表达F4/80高、SiglecF低、CD11c低•高表达CXCR4、MHC-II、CD101和CD319•低表达中性粒细胞活化标志物CD177 3、形态学特征,通过离心涂片和吉姆萨染色观察,Ly6G+巨噬细胞呈现以下特点: •肾形细胞核(类似炎性单核细胞)•细胞质中含有大量空泡状结构•细胞膜富含突起 ...
•表达F4/80高、SiglecF低、CD11c低•高表达CXCR4、MHC-II、CD101和CD319•低表达中性粒细胞活化标志物CD177 3、形态学特征,通过离心涂片和吉姆萨染色观察,Ly6G+巨噬细胞呈现以下特点: •肾形细胞核(类似炎性单核细胞)•细胞质中含有大量空泡状结构•细胞膜富含突起 ...