Instead, IRF-4f/fCD11c-cre mice have fewer CD11b+migrating DCs and fewer DCs carrying parasite antigens to the lymph nodes. Furthermore, CD11b+CD103+DCs induce Th2 responses in the small intestine, whereas CD11b+CD103DCs perform this role in the colon, revealing a specific functional ...
we generated a mouse strain in which Ly-6C+cDC2 represent the vast majority of cDCs in the lung. We first bred mice carrying a Cre recombinase-inducible gene encoding the diptheria toxin alpha subunit (DTAfx)
cellsjnthespinalcordsoftheEAEmice.LFBstainingrevealedmultipledemyelinatedIesionsintheaboveinflammatory infiltration.TheimmunofluorescenthistochemistrysuggestedthatmanyG卜lCD11b MDSCswerepresentinspinalcordsofthe EAEmice.butnopos itiv e ce lls we re obs e rved in the ce ntre l g roup.The ma ss of...
The absence of T and B cells did not dampen BRBs' anti-tumor effects in the NOD.SCID mice. In the Kras LSL.G12D/+ -Trp53 LSL.R172H/+ -Pdx-1-Cre mice, BRBs significantly prolonged survival (189 days versus 154 days). In both models, BRBs decreased tumor-infiltrating CD11b+ ...
T-cell-dependent inflammation in CD11c-Cre.Tgfbr1fl/flmice To explore the role of TGFβ in intestinal DC development, we crossedTgfbr1fl/flmice withItgax-Cre mice, which constitutively express Cre recombinase under control of the CD11c promoter25(referred to here as CD11c-Cre). Although CD11...
In tumor-bearing mice, IL-33 induced substantial accumulation of degranulating eosinophils within tumor necrotic areas, indicating cytotoxic activity in vivo. Blocking of CD11b/CD18 signaling significantly reduced IL-33-activated eosinophils' binding and subsequent killing of tumor cells, indicating a ...
Using both FRET mice, we were able to determine that LFA-1 and Mac-1 distinctly regulate the neutrophil extravasation cascade. Our data suggest that the vascular endothelium functions as a double- layered barrier in the steps of neutrophil extravasation. We propose that the harmonized regulation ...
A syngeneic PDAC cell line (KP2) derived from the genetic p48-Cre;LSL-KrasG12D;Trp53flox/+ mice was orthotopically implanted in wild-type (WT) and CD11b-null mice. As expected, treatment of established tumors with GB1275 repressed PDAC progression, decreased TAM infiltration, and increased...
2a–c). MRP8-Cre;Cd47fl/fl mice developed normally, and importantly, no differences were found with littermate controls in the size and appearance of the small and large intestine as well as spleen. Furthermore, MRP8-Cre;Cd47fl/fl mice did not exhibit signs of spontaneous intestinal ...
为了验证这一发现,研究者利用Cre-loxP系统特异性敲除了MrgprD+神经元中的δOR。结果显示,这些小鼠在SNI模型中表现出更严重的疼痛行为,并且对δOR激动剂和IL-2处理均无反应。这一结果表明,δOR是mTreg细胞通过内啡肽发挥镇痛作用的关键受...