-w INT minimizer window size [51] -f INT number of bits for bloom filter; 0 to disable [37] -D FLOAT drop k-mers occurring >FLOAT*coverage times [5.0] -N INT consider up to max(-D*coverage,-N) overlaps for each oriented read [100] -r INT round of correction [3] -z INT len...
Pacbio Sequel II平台的下机数据为bam格式, bam文件可直接适配大多数的下游分析软件,存储有效数据的文件一般命名为:*.subreads.bam,*.subreads.bam.pbi。 输入文件:sample.subreads.bam以及相对应的索引sample.subreads.bam.pbi 输出文件:unaligned BAM (.bam);bgzipped FASTQ (.fastq.gz)。 基础使用,全部参数...
Output:Consensus reads in a format inferred from the file extension: unaligned BAM (.bam); FASTQ (.fastq); or SMRT Link XML (.consensusreadset.xml) which also generates a corresponding BAM file. 用以下代码跑 Run on a full movie:
OUT.ccs.bam|fastq.gz|xml FILE Consensus reads (.bam, .fastq.gz, or .consensusreadset.xml). Input Filter Options: --min-passes INT Minimum number of full-length subreads required to generate CCS for a ZMW. [3] --min-snr FLOAT Minimum SNR of subreads to use for generating CCS [2.5]...
Pacbio Sequel II平台的下机数据为bam格式, bam文件可直接适配大多数的下游分析软件,存储有效数据的文件一般命名为:*.subreads.bam,*.subreads.bam.pbi。 输入文件:sample.subreads.bam以及相对应的索引sample.subreads.bam.pbi 输出文件:unaligned BAM (.bam);bgzipped FASTQ (.fastq.gz)。
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