品系描述 This Ccr2 knockout mouse model was generated by knocking out the exon 3 region of the Ccr2 . 应用领域:Immune-related *使用本品系发表的文献需注明: Ccr2-KO mice (Cat. NO. NM-KO-190018) were purchased from Shanghai Model Organisms Center, Inc.. ...
CCR2 KO mice also expressed higher levels of the osteoclastogenic and osteolytic factors, receptor activator of nuclear factor kappa B ligand (RANKL) and cathepsin K, of the proinflammatory cytokine tumor necrosis factor鈥揳lpha, and of the neutrophil migration related chemokine, KC. These results...
Panel g shows mean values obtained from the Peyer's patches (PP), lamina propria (LP), and intraepithelial lymphocyte (IEL) compartment in a representative experiment using three mice per strain (*P < 0.05). KO, knockout. infiltration into the lamina propria, and modest thickening of the ...
This results in a significantly reduced number of basal and inflammation-induced circulating classical, and even non-classical monocytes in Ccr2 knockout (Ccr2-KO) mice16,17. Consequently, Ccr2-KO mice are often utilized to study the role of infiltrating monocytes in disease models. In this ...
C57BL/6.CCR2-KO (CCR2-/-) were kindly provided by W.A. Kuziel (University of Texas, Austin, TA).21 Mice used in bone marrow transplantation (BMT) experiments were between 8 and 12 weeks old. Bone marrow (BM) cells were isolated by flushing femurs and tibias. Donor BM was T-cell...
To determine PSMP chemo-attracted monocytes in a CCR2-dependent manner, we performed adoptive transfer of CD11b+Ly6Chibone marrow cells from the wild type mice (CD45.1) to CCR2 KO mice (CD45.2) (Fig.2A). The cells were administered intravenously into CCR2 KO recipients coincident with injec...
CCL2/CCR2 gene knockout mice and macrophage-specific PPARG gene knockout mice were respectively used to investigate the role of infiltration and polarization of TAMs in ESCC. CCL2-mediated monocyte chemotaxis was estimated in malignantly transformed Het-1A cells. THP-1 cells were used to ...
Unfortunately, mice cannot be used to explore the tissue expression and biological functions of CCR2A because the mouse CCR2 gene does not undergo the alternative splicing process necessary to generate CCR2A. The alternative splicing of the human CCR2 gene produces two proteins with different C-...
Likewise, CCR5 -/- mice are normal, unless stressed. However, if human CCR5Δ32 homozygotes like CCR5 -/- mice are more susceptible to listeria, cryptococcus, cerebral malaria or other infections, this may provide a balancing positive selection pressure to retain CCR5 (Zhou et al., 1998;...
gondii infection, infected E-Atg5 KO mice demonstrated a further increase in TNF levels compared to WT mice infected with the parasite (Figure 2K). Combined with the previous reports describing a major role for TNF in intestinal inflammation (Egan et al., 2009; Liesenfeld, 2002; Liesenfeld et...