Fig 3F-J:由于很多心脏细胞都表达MyD88,作者cross了B6 MyD88–floxed mice 和 B6 LysM-Cre,得到组织原位巨噬细胞特异性MyD88缺陷鼠(尽管髓系细胞都表达LysM,但心脏没有组织原位中性粒)。作者将得到的组织原位巨噬细胞MyD88缺陷鼠的心脏移植给syngeneic LysM-GFP recipients,得到了和前面一样的结果。 随后作者想要...
通过在Ccr2基因目的exon 3两侧分别插入loxP位点。该flox小鼠可与组织特异性Cre工具鼠交配,获得在特定细胞类型或组织中敲除Ccr2基因的小鼠模型。 *使用本品系发表的文献需注明: Ccr2-Flox mice (Cat. NO. NM-CKO-226213) were purchased from Shanghai Model Organisms Center, Inc.. ...
本研究利用嗜神经病毒(JEV,HSV-1,ZIKV)感染小鼠发现:病毒感染的脑组织巨噬细胞VEGFR-3特异性上调表达(在DCs,T细胞和NKs中不上调),且脑中其配体血管内皮生长因子C(VEGF-C,并非VEGF-D)的分泌量增加.JEV感染的VEGFR-3激酶抑制剂处理的小鼠或髓系细胞(LysM-Cre) VEGFR-3配体结合域(LBD)特异性缺失小鼠(Vegfr-3...
These cells extravasate from the bloodstream through a process mediated by chemokines se- creted from resident cells. Chemokines are a family of che- motactic cytokines that induce the migration of various cell types; to date, 440 chemokines have been identified.9 Among these, monocyte ...
PPARγloxP mice were bred with Lyz2cre mice to generate macrophage-specific PPARG deletion mice (PPARG−/−ΔMacrophage). The mice were bred at the Shanghai Model Organisms Center (Shanghai, China) and used between the ages of 4 and 8 weeks. The ESCC rat model [19, 20] and ...
为了证实这些发现,作者检测了来自KPC基因工程模型的另一个同源PDAC细胞系的原位肿瘤(p48CRE/LSL-KrasG12D/p53flox/flox),发现CXCR2i也阻止了模型中TAN的积累(在线补充图S3) 最后表明CXCR2i或LY6G耗竭对肿瘤重量的作用 在建立的KPC疾...
Eomes deficiency in CD4 þ T cells using B6.Cd4CreEomesfl/fl-mixed BM chimeras reduced Th17 cell generation in a T-cell intrinsic manner (Fig. 6f), identifying Eomesodermin as a novel regulator of Th17 cell development in vivo. Strikingly, despite abundant a 3 day ex vivo culture MOG35...
为了研究FGF12在肝纤维化中发挥的作用,作者构建了髓系特异性FGF12敲除小鼠(Fgf12 fl/fl;Lyz2cre+/−)以敲除巨噬细胞中的FGF12。同窝Fgf12 fl/fl小鼠作为对照。然后作者对这些小鼠进行BDL,天狼星红染色显示Fgf12 fl/fl;Lyz2cre+/...
为了证实这些发现,作者检测了来自KPC基因工程模型的另一个同源PDAC细胞系的原位肿瘤(p48CRE/LSL-KrasG12D/p53flox/flox),发现CXCR2i也阻止了模型中TAN的积累(在线补充图S3) 最后表明CXCR2i或LY6G耗竭对肿瘤重量的作用 在建立的KPC疾病模型中, 作者用CXCR2i或LY6G特异性耗尽抗体治疗,发现与对照组相比,肿瘤重量减...
As we found previously, CD11c-cre x IRF4fl/ þ mice had a substantial decrease in the numbers and proportions of CD103 þ CD11b þ DCs in SI LP and, interestingly, these animals also showed a significant defect in the CCR2 þ subset of CD103 À CD11b þ DCs (Figure 6b)....