大多数大肠杆菌克隆菌株不含有F质粒(被称为F-);但有一些常见的实验室菌株是F'的,如NEB Stable、JM109、XL1和XL10 Gold,这些菌株可用于繁殖或保存含ccdB的空质粒骨架,但对重组质粒进行筛选时不能使用。 参考书籍:Plasmids 101:A Desktop Resource —— Created and Compiled by Addgene March 2017(3rd Edition)...
产品简介 基因名CC2D1B 英文全称coiled-coil and C2 domain containing 1B 别称KIAA1836 中文名称含coiled-coil 和C2结构域蛋白1B UniprotQ5T0F9 GeneID200014 交叉反应(推测)Human Mouse Rat 氨基酸大小17kDa 表达氨基酸1-159aa BackgroundTranscription factor that binds specifically to the DRE (dual repressor...
6.权利要求1～5任一所述试剂盒的使用方法,其特征在于,包括如下步骤:(1)设计目的基因扩增引物gene-HF与gene-HR,其序列均包括2个片段:(I)用于扩增目的基因的片段,(II)用于与表达载体同源重组的片段;片段(I)位于片段(II)的3’侧;所述同源重组的片段是载体扩增引物与表达载体配对的部分或全部片段;(2)使用HF和...
Lund BA,Leiros HKS,Bjerga GEK.A high-throughput,restriction-free cloning and screening strategy based on ccd B-gene replacement[J].M icrobial Cell Factories,2014,1:38-38.A high-throughput,restriction-free cloning and screening strategy based on ccd Bgene replacement. Lund B A,Leiros H K S...
如果您对克隆感兴趣,您可能知道目前有几种方法可以用来进行克隆。其中包括传统的限制性内切酶/连接酶介导的方法、最近开发的Gibson Assembly Cloning和Gateway® 克隆技术,以及其他几种技术。每种方法都是独一无二的,并依赖于对克隆反应至关重要的特定组件。了解特定成分对于为您自己的实验选择正确的克隆方法至关重要,...
All Donor vectors and Destination vectors contain the ccdB cell death gene to reduce background of non-recombined BP/LR plasmids. Therefore, growing non-recombined vector requires special cells (One Shot ccdB Survival 2 T1R Competent Cells) which are resistant to the lethal effects of ccdB. ...
a、PCR扩增ccdB基因表达盒子、表达载体和目的基因,得到含线性ccdB基因表达盒子的PCR产物、含线性表达载体的PCR产物和含线性目的基因的PCR产物;所用到的引物为:(1)ccdB基因表达盒子的扩增引物ccdB-HF和ccdB-HR;(2)载体扩增引物HF和HR;(3)扩增目的基因的引物gene-HF和gene-HR; ...
vectors using the F plasmid ccdB killergene[J]. Gene, 1994, 148(1):71-74. [15] 陈其军, 王学臣, 周海梦. 一种构建T 载体的方法[P]. 中国专 利: 1590549A, 2009-09-01. [16] 顾頠, 张昕, 安小平, 等. 基于XcmⅠ酶切的高效TA克隆载体 ...
CCD has been mapped to chromosome 6p21, where CBFA1, a gene encoding OSF2/CBFA1, a transcriptional activator of osteoblast differentiation, has been ... B Lee,K Thirunavukkarasu,L Zhou,... - 《Nature Genetics》 被引量: 2046发表: 1997年 Tau is a candidate gene for chromosome 17 front...
One Shot®ccdB Survival™ 2 T1RChemically Competent Cells are suitable for the propagation of plasmids containing theccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation ef...