The sequence in brackets comes from attB, and N is your gene-specific sequence. Note: When creating an Entry Clone through the BP reaction and a PCR product, the vector backbone is not the same as Gateway Entry vectors. The backbone in the case of PCR BP cloning is pDONR201. 1. Yeast...
We observe an interplay of numerous factors, namely, location of the codon, codon usage, t-RNA abundance, formation of anti-Shine Dalgarno sequences, predicted transcript secondary structure, and evolutionary conservation in determining phenotypic effects of ccdB synonymous mutations. Incorporation of ...
cholerae fur gene probes to demonstrate that there are fur-homologous sequences in the DNAs of V. vulnificus, Vibrio fischeri, and ... C.M. Litwin,S.B. Calderwood - 《Journal of Bacteriology》 被引量: 167发表: 1993年 H-NS Protein Represses Transcription of the lux Systems of Vibrio ...
Mature B cells will typically expresseither immunoglobulin M (IgM) or immunoglobulin D (IgD) on their surface,邻家的IgD妹妹IgM,天真少女(IgM,IgD)andthis surface expression is critical to cell survival. Further, the combinationof the VDJ gene sequences is un...
图3虫体18S rRNA基因序列Fig. 3The sequence of 18S rRNA gene ofSparganosis mansoni 2.3 BLAST比对分析结果 将所获得序列(图3)在GenBank中进行BLAST比对分析,发现所获序列与GenBank中所公布的曼氏迭宫绦虫18S rRNA基因序列的相似度为100%。由此确认,该虫为曼氏裂头蚴,确诊...
A method for knocking out bovine beta-lactoglobulin gene by using zinc finger nucleases is provided, in which zinc finger nucleases (ZFNs) specific binding domains targeting the specific sequences in bovine beta-lactoglobulin gene are designed according to the sequence of the gene and the expression...
2004. Phylogenetic analysis and PCR-restriction fragment length polymorphism identification of Campylobacter species based on partial groEL gene sequences. J. ... RI Karenlampi,TP Tolvanen,ML Hanninen - 《Journal of Clinical Microbiology》 被引量: 0发表: 2005年 Phylogenetic Analysis and PCR-Restrict...
Curcumin has demonstrated valuable therapeutic potential against a variety of human cancers including osteosarcoma. However, the molecular mechanisms underlying its anti-tumor effect remain to be poorly understood. By RNA sequence profiling, we found that curcumin significantly down-regulates the expression ...
470. sequence n. 连续;顺序 471. shallow a. 浅的 472. shiver vi/n. 发抖 473. shrug v./n. 耸肩 474. signature n. 签名 475. sincere a. 诚挚的,真诚的 476. utility n. 功用,效用 477. utilize vt. 利用 478. utter vt. 说出 a. 完全...
Changes in the plant growth or microspore culture conditions were not by themselves sufficient to induce napin gene expression. Specific members of the napin multigene family were cloned from a cDNA library prepared from microspores that had been induced to undergo embryogenesis. The majority of ...