Furthermore, we aimed to prove that SDF-1α cleavage by CatK inactivates SDF-1α and inhibits the invasion of GSLCs. We performed mass spectrometric analysis of cleavage products of SDF-1α after proteolysis by CatK. We demonstrated that CatK cleaves SDF-1α at 3 sites in the N-...
Site Map Biomedical Research Service & Clinical Application Alphabetical Listing HepG2 cells were treated with MG132 to induce autophagy. The CTSC assay shows the induction of cathepsin C activity during autophagy.
A. OverviewApoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that ...
The SensoLyte® 520 Cathepsin G Activity Assay Kit uses a HiLyte™ Fluor 488/QXL™ 520 FRET peptide substrate for the measurement of enzyme activity. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL™ 520. Upon cleavage of the FRET peptide by th...
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used...
The cleavage occurs at the Phe-Phe amide bond resul. Cathepsin D and E FRET Substrate is a valuable tool for routine assays and for mechanistic studies on cathepsins E and D[1]. 分子量 1816.15 Formula C87H126N22O21 Sequence MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-{Lys(Dnp)...
Moreover, a cathepsin B cleavage assay confirmed the desired enzymatic activation of the prodrug. Together, the obtained data show that the concept of cathepsin Bヽleavable prodrugs can be transferred to the class of targeted therapeutics, allowing the development of optimized tyrosine kinase ...
Although the binding would not be the same as in cathepsin B, due to the lack of an equivalent to His111, both cleavages (mono and di) would occur [5]. Finally, in order to confirm the presence of cathepsin B in the spine extract, we performed a Western blotting assay. Bands ...
Like its mammalian counterpart, chicken cathepsin D is likely to be secreted as an inactive form, which is further processed into an active intermediate by autocatalytic cleavage of its propeptide (Retzek et al., 1992). The proteases that are responsible for the processing of chicken cathepsin D...
Pepstatin inhibited proteolysis almost completely, as measured by either assay. Leupeptin decreased hydrolysis to acid-soluble products by approximately 50%, but had no effect on the initial cleavage of intact apoprotein B. Similar results were found in the case of extracts from cultured human skin ...