1 × 105 cells/well were added for 24 h to earlier prepared DQ gelatine-coated dishes in full medium supplemented with or without MMP-2 inhibitor ARP101, Furin inhibitor I or Cathepsin B inhibitor, CA-074ME. FITC fluorescence generated by the cleavage of DQ gelatine was measured using a ...
forming function and ADP-ribosyltransferase activity. Protein binding assay suggests that MAV_4644 interacts with the host lysosomal peptidase cathepsin Z (CTSZ), a key regulator of the cell signaling and inflammation. Pathogenic mycobacteria have been shown to suppress the action of many cathepsins ...