Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also ...
Moreover, a cathepsin B cleavage assay confirmed the desired enzymatic activation of the prodrug. Together, the obtained data show that the concept of cathepsin Bヽleavable prodrugs can be transferred to the class of targeted therapeutics, allowing the development of optimized tyrosine kinase ...
Although the binding would not be the same as in cathepsin B, due to the lack of an equivalent to His111, both cleavages (mono and di) would occur [5]. Finally, in order to confirm the presence of cathepsin B in the spine extract, we performed a Western blotting assay. Bands ...
In case of chromogenic or fluorogenic peptidic substrates, the kinetic parameters of substrate consumption have been used to perform active site mappings of cathepsin B. Following this approach, an accelerated cleavage of defined peptides enables the identification of advantageous interactions between the ...
The action of cathepsin S on the oxidized B chain of insulin is very similar to that of cathepsin L with one exception: the Tyr26-Thr27 bond is resistant to cathepsin S, even after 10 h incubation [3]. Different cleavage products have been evaluated by proteolysis of thyroglobulin [20] ...
mechanismsotherthanthetraditionalcaspase-mediatedcleavagecascade. Thereisgrowingrecognitionthatalternativeproteolyticenzymessuchasthelysosomalcathepsinproteases mayinitiateorpropagateproapoptoticsignals.Cathepsinsarelysosomalenzymesthatarealsousedas sensitivemarkersinvarioustoxicologicalinvestigations.TheCathepsin-BActivityAssaykit...
However, truncated synuclein isoforms generated by catB cleavage have an increased propensity to aggregate. Thus, catB activity could potentially contribute to lysosomal degradation and clearance of pathogenic alpha synuclein from the cell, but also has the potential of enhancing synuclein pathology by ...
摘要: ICT's Magic Red(R) Cathepsin assay kits enable researchers to quantitate and monitor intracellular cathepsin B, K, or L activity over time in vitro. The Magic Red(R) (MR) reagent is a non-cytotoxic substrate that fluo-resces red upon cleavage by active cathepsin enzymes....
(B) Numbers of GSLCs invading into Matrigel out of spheroids were determined using image analysis after treatment with SDF-1α, PepC-C, cleavage products of SDF-1α generated by CatK at pH 5.5 and 7.4 (< 3 kDa) and medium only as control. All invaded cells, including the cells that ...
They concluded that potato proteins possess substantial reducing power that can be attributed to peptide cleavage, which results in a product that has a higher availability of hydrogen ions. These hydrogen ions are able to transfer and stabilize the free radicals to a greater extent from the ...