Intracellular lipopolysaccharide from Gram-negative bacteria including Escherichia coli, Salmonella typhimurium, Shigella flexneri, and Burkholderia thailandensis activates mouse caspase-11, causing pyroptotic cell death, interleukin-1β processing, and
Caspase-11 gene expression in response to lipopolysaccharide and interferon-gamma requires nuclear factor-kappa B and signal transducer and activator of transcription (STAT) 1. J Biol Chem. 2002;277:41624–30. Article CAS Google Scholar Ricardo SD, van Goor H, Eddy AA. Macrophage diversity in ...
活化的Caspase-4、5、11切割GSDMD,形成含有GSDMD氮端活性域的肽段,诱导细胞膜穿孔,细胞破裂,释放内容物;同时活化的Caspase-4、5、11在NLRP3和ASC存在下可与Caspase-1相互作,从而促进其激活,介导下游IL-1β和IL-18的成熟和释放[13-14]。
The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme[J]. Cell, 1993, 75(4): 641-652. [11]SCOTT AM, SALEH M. The inflammatory caspases: Guardians against infections and sep...
起始组Caspase包括caspase-2,-8,-9,-10,-11和-12,与促凋亡信号紧密相连,一旦激活,这些酶会切割并激活下游的效应组Caspase,包括Caspase-3,-6,-7。效应 Caspase通过对细胞内蛋白特定的天冬氨酸残基位置处进行切割实现细胞的凋亡。FasL和 TNF对Fas和 TNFR的结合能够激活caspase-8和-10。DNA损伤诱导PIDD的表达,...
通常是细胞质脂多糖(lipopoly-saccharide,LPS)直接激活Caspase-4、Caspase-5和Caspase-11,活化的Caspase-4、5、11切割Gasdermin D产生与Caspase-1相同的裂解作用,从而导致细胞膜穿孔。 细胞焦亡与细胞凋亡相似,与细胞凋亡不同的是,细胞焦亡主要是由caspase-1激活的。因此检测caspase-1活性是细胞焦亡分析的关键。 美国...
a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms...
2019年11月14日,来自美国Genentech公司的Vishva M. Dixit研究组在Nature杂志上发表题为Activity of caspase-8 determines plasticity between cell death pathways的研究文章,发现肠道上皮细胞的CASP8(C362A)通过活化ASC,活化蛋白酶caspase-1,从而诱导细胞死亡,而RIPK3同样参与这一现象。 由多项报道指出,在癌症细胞中...
1H11 抗原 Purified recombinant fragment of human CASP8 expressed in E. Coli. 偶联物 Unconjugated 形式 Liquid 浓度 Conc. Not Determined 保存液 ascites 内含物 0.03% sodium azide 保存条件 Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. ...
Human Caspase-12 is located in the same gene cluster as COPs, on chromosome 11q23, and is expressed as a full-length or truncated protein. While the full-length Caspase-12L has only been identified in 20% of the population, particularly in individuals of African descent, the truncated Caspa...