CRISPR-Cas9蛋白-SPCas9-NG是一种RNA介导的核酸内切酶,可以催化双链DNA的特异位点切割。其识别 PAM(Protospacer Adjacent Motif)序列的NG处。 CRISPR Cas9蛋白的结构域。CRISPRCas9蛋白由六个结构域组成。晶体结构、图示来自PDB文件: 4CMP 【CRISPR Cas9蛋白用途】 ...
Magigen CRISPR Cas9蛋白 | SPCas9-NG 70 CRISPR-Cas9蛋白SPCas9-NG 是一种 RNA 介导的核酸内切酶,可以催化双链 DNA 的特异位点切割。其识别 PAM(Protospacer Adjacent Motif)序列的NG处。 CRISPR Cas9蛋白的结构域。 CRISPR-Cas9蛋白由六个结构域组成。晶体结构、图示来自PDB文件: 4CMP 【CRISPR Cas9蛋白用途】...
2018年报道的一个SpCas9变体,SpCas9-NG(R1335V/L1111R/D1135V/G1218R/E1219F/A1322R/T1337R)可将NGG PAM扩宽至NG PAM, 并对NGT, NGG和NGA PAM仍保持较高活性,有效地缓解PAM限制性问题 (Nishimasu et al., 2018)。 在植物中,已有多个独立报道利用SpCas9-NG构建基因组编辑和碱基编辑工具,实现了基因组...
Here, we evaluated the nuclease activity of Cas9-NG toward various NGN PAMs by targeting endogenous genes in transgenic rice, and revealed that Cas9-NG edits all NGG, NGA, NGT and NGC sites with impaired activities, and gene-edited plants were dominated by mono-allelic mutations. The Cas9-NG...
SpCas9-NG is a SpCas9 variant that can recognize relaxed NG PAMs. 【Catalog#】 C003X 【Introduction】 With its pure protein system that resists distraction and low off-target rate, The CRISPR-Cas9 technologies is a safe and common method in the field of gene editing. ...
Streptococcus pyogenes Cas9 (SpCas9)-NG recognizes NGN protospacer adjacent motifs and expands the scope of genome-editing tools. In this study, we found that SpCas9-NG not only targeted the genome but also efficiently self-targeted the single-guide RNA
化学名:pCMV-T7-SpCas9-NG-P2A-EGFP(RTW3677) 别名: CAS番号: 分子式: 分子量:0 EINECS: Mol File:Mol File Browse by Nationality SuppliersJAPAN suppliers China (1)Member(1)All (1) Recommend You Select Member Companies. 名前:Beijing Solarbio Science & Tecnology Co., Ltd. ...
西湖云谷智药基因编辑工具AIdit_SpCas9-NG活性预测软件是由西湖云谷智药(杭州)基因科技有限公司著作的软件著作,该软件著作登记号为:2023SR0813690,属于分类,想要查询更多关于西湖云谷智药基因编辑工具AIdit_SpCas9-NG活性预测软件著作的著作权信息就到天眼查官网!
通常使用的CRISPR/Cas9系统仅能识别靶标序列为NGG的原间隔序列临近基序(protospacer adjacent motif,PAM),而进化的xCas9,SpCas9-NG系统可识别序列为NG的PAM,扩大了 靶... 张宁,秦怀远,辛京京,... - 《农业生物技术学报》 被引量: 0发表: 2021年 SpCas9酶切机制初探及其在生物合成基因簇克隆的应用 成簇规律间...
Product Name:pCMV-T7-SpCas9-NG-P2A-EGFP(RTW3677) Synonyms: CAS: MF: MW:0 EINECS: Mol File:Mol File Browse by Nationality Suppliers >Global suppliers Beijing (1) Member (1) All (1) EnquiryPlease select the suppliers Recommend You Select Member Companies Company Name: Beijing Solarbio...