2024年11月19日,荷兰莱顿大学医学中心Erik R.Abels团队在MolecularTherapy杂志在线发表了题为“CRISPR targeting of mmu-miR-21a through a single adeno-associated virus vector prolongs survival of glioblastoma-bearing mice”的研究论文,利用单个腺相关病毒载体AAV8携带SaCas9(金黄色葡萄球菌的Cas9同源蛋白)的变体Sa...
However, these tumors effectively grew in syngeneic C57BL/6J immunodeficient, T cell-depleted and Cas9-KI mice. Therefore, these observations suggest that this tumor rejection phenotype is T cell-dependent. The immunological clearance of SpCas9-expressing tumors in the immunocompetent group illustrates...
品系描述 通过C57BL/6-Gt(ROSA)26Sortm1(CAG-LNL-Cas9)Smoc(NM-KI-00038)与EIIA-Cre工具鼠交配得直接全身表达Cas9蛋白的小鼠。 应用领域:基因敲除、基因修饰 *使用本品系发表的文献需注明: R26-CAG-Cas9 mice (Cat. NO. NM-KI-00120) were purchased from Shanghai Model Organisms Center, Inc.. ...
CRISPR has been used to generate genetically modified mouse models such as KO/KI germline models, somatic genome editing models and mouse models for drug treatment. CRISPR has been proved a useful tool to investigate chromosomal engineering, generate ex vivo leukemia models and identify drug ...
In this chapter, we provide a step-by-step protocol to obtain Knock-Out (KO ) or Knock-In (KI ) mouse models using CRISPR /Cas9 technology. Detailed instructions for the design of single guide RNAs (sgRNA ) for KO approaches and single-strand oligonucleotide (ssODN ) matrix for generation...
CAR-T cells were more efficacious in IL-2 transgenic mice, helping CAR-T cells resist the suppressive immune environment and finally getting durable antitumoral responses in humanized mouse models [99]. IL-23 is a two-subunit cytokine, including the p19 and p40 subunits, known to promote the...
*使用本品系发表的文献需注明: R26-CAG-LNL-Cas9-IRES-Luc mice (Cat. NO. NM-KI-18002) were purchased from Shanghai Model Organisms Center, Inc.. 发表文献 Balance of Gata3 and Ramp2 in hepatocytes regulates hepatic vascular reconstitution in postoperative liver regeneration ...
Cyagen has analyzed over 3,400 publications by our customers, comparing the projects that use either of these two technologies (ESC Gene Targeting and CRISPR-Pro) in a variety of models:conditional knockout (cKO), knockin (KI), secondary targeting, humanization, conditional point mutation, KO-Fi...
Delivering the CRISPR/Cas9 system to treat cancer, liver fibrosis, obesity, and cardiovascular diseases.aAPACPs, exosomes, PICASSO and CHO-PEGA were intravenously injected into mice, and AAV9 was intraperitoneally injected.bAPACPs are transported through the blood circulation to the tumor tissue, and...
统计 F2 代中野生(Wt)、杂合和敲入(KI)3 种基因型小鼠的存活数量。选取 F2 代同窝 Wt 和 KI 雄性小鼠(8 只 / 组)给予普通饮食 8 周,每周记录小鼠的体质量,检测两种小鼠葡萄糖代谢和脂质代谢等指标。组间比较采用独立样本 ...