此外，这个在线工具也可以设计cas13的gRNA:https://cas13design.nygenome.org/cas13design.nygenome...
Cas13d的guide RNA设计可以参考这个网站Cas13design (nygenome.org)，首页有一些参考文献可以读一下。
(https://gitlab.com/sanjanalab/cas13). Moreover, we provide precomputed gRNA predictions targeting all protein-coding transcripts in the human genome on our web-based repository (https://cas13design.nygenome.org). Other data and materials that support the findings of this research are ...
A major challenge in the application of the CRISPR-Cas13d system is to accurately predict its guide-dependent on-target and off-target effect. Here, we perform CRISPR-Cas13d proliferation screens and design a deep learning model, named DeepCas13, to pred
The performance assessment of the engineered systems also revealed guide RNA design principles. Moreover, we show that the system can work in vivo. This development complements the ability to regulate transcription with other CRISPR-Cas systems and offers potential applications. 展开 ...
Similar to the gRNA in the CRISPR/Cas9 system, the crRNA used by Cas13 forms a short hairpin structure next to a short spacer sequence (28–30 nucleotides) that is specific to the target transcript (Fig. 1d). Since CRISPR/Cas13 mediates RNA degradation, it holds the promise to replace ...
Construction of an optimal gRNA for translational repression Given that gRNAs may have differential efficacy of target cleavage18,24,80,82,83, we conducted a systematic survey to identify a gRNA design effective for translational inhibition. We tested the minimal length of the spacer region. Since ...
CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various organisms. However, collateral degradation of bystander RNAs has limited their in vivo applications. Here, we design a dual-fluorescence reporter system for detecting collateral effects and screening Cas13 variants in...
design, each cell stably expresses a specific gRNA that upon induction of Cas13 also integrated into the genome can induce knockdown of the target transcript. Thus, each cell is barcoded by a unique gRNA sequence. The effect of the knockdown on cellular viability could then be judged by ...