For IP, the extracts were incubated with 4 μg control IgG or anti-CARM1 (Cell Signaling, #3379S) antibody for 3 h at 4 °C followed by incubation with protein A + G Dynabeads for another 1 h. After incubation, the beads were washed with buffer A containing 150 mM ...
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ASMMC7721 cells were transfected with FLAG-TRIM47 for 48 h. Cell extracts were subjected to immunoprecipitated with anti-FLAG antibody or control IgG. Arginine methylation of immunopurified TRIM47 was detected by western blot.BCARM1 stably knockdown (shCARM1#1) or control (shNC) SMMC7721 cell...
BAF155 (D7F8S) Rabbit Cell Signaling 49926, RRID:AB_10950224 Total Histone H3 (C-16) Santa Cruz Sc-8654, RRID:AB_2118303 Mono-methyl SmB Sigma Aldrich S0698, RRID:AB_2193867 Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Cell Signaling #3505, RRID:AB_2298971 Anti-PABP antibody [...
An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
Anti-PKM2 (4053) and anti-PKM1 (7067) antibodies were purchased from Cell Signaling Technology. Anti-CARM1 (A300–421A) antibody was purchased from Bethyl Laboratories. Plasmids and siRNA Human PKM1 (44241)- and PKM2 (44242)-expressing constructs for His-tagged recombinant protein purification ...
An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
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An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
Cancer cells autonomously alter metabolic pathways in response to dynamic nutrient conditions in the microenvironment to maintain cell survival and proliferation. A better understanding of these adaptive alterations may reveal the vulnerabilities of canc