CARM1 Antibody (A300-421A) in WB Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody A300-...
CARM1 Antibody (A300-421A) in WB Detection of human CARM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CARM1 antibody A300-...
CARM1 (Ab-228) Antibody 供应商 MyBioSource 产品货号 MBS857509 产品报价 ￥询价/0.05mg ￥询价/0.1mg ￥询价/其它规格 产品说明书 点击查看 购买方式 MyBioSource中国区金牌代理,部分产品现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868 ...
英文名字 Phospho-CARM1-S228 Polyclonal Antibody供应商 MyBioSource产品货号 MBS128261产品报价 ￥询价/0.05mL ￥询价/0.1mL ￥询价/其它规格产品说明书 点击查看购买方式 MyBioSource中国区金牌代理,部分产品现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868产品新闻...
Total cell lysates from transfected cells were separated by gel filtration, followed by Western blot analysis using an anti-FLAG antibody to determine the proportion of mono-, di-, and tetramers (indicated with open circles above the gels). D, reduced PKM2 tetramerization in Carm1−/− MEF...
An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
E15S and E11S do not affect the CARM1 protein level, which was confirmed by transforming them into HEK293T cells and detecting the translated products with western blotting using anti-HA antibody (Extended Data Fig.2e). Second, E15S and E11S are not specific for two-cell and four-cell...
Anti-PABP antibody [10E10] Abcam Ab6125, RRID:AB_2156878 GAPDH FL-335 Rabbit Santa Cruz Sc-25778, RRID:AB_10167668 Donkey Anti-Mouse IgG 680RD LI-COR- Biosciences 926-68072 RRID:AB_10953628 Donkey Anti-Rabbit IgG 680RD LI-COR- Biosciences 926-68073, RRID:AB_10954442 Donkey Anti-Mouse ...
The antibody-chromatin complexes were subsequently pulled down using protein A sepharose beads, followed by extensive washing with high salt, lithium chloride, and TE buffer. Elution of the complexes was performed, and crosslinking was reversed in a water bath at 65 °C for 4 h. Proteinase...
CARM1 was immunoprecipiated with the anti-FLAG antibody, and the presence of BAF155, MED12, PABP1, NCOA3 and TET2 in the immunoprecipitates was detected with western blots using the respective antibodies. The loading controls are depicted below the corresponding western blot results, separately ...