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Chromatin immunoprecipitation The following antibodies were used to perform ChIP: anti-H3K27Me3 (Cell Signaling, Cat. No: 9733), anti-BAF155 (Santa Cruz, Cat. No: sc-9746), anti-RNA polymerase II (Santa Cruz, Cat. No: sc-899), or anti-EZH2 (Cell Signaling, Cat. No: 5246). An i...
Total Histone H3 (C-16) Santa Cruz Sc-8654, RRID:AB_2118303 Mono-methyl SmB Sigma Aldrich S0698, RRID:AB_2193867 Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) Cell Signaling #3505, RRID:AB_2298971 Anti-PABP antibody [10E10] Abcam Ab6125, RRID:AB_2156878 GAPDH FL-335 Rabbit Santa...
(ab12162) and anti-H3R17me2 (ab8284) (Abcam); anti-acetyl-Histone H4 (06-866) (Millipore); anti-IgG (sc-2025) (Santa Cruz Biotechnology). The antibody specifically recognizing arginine methylated Pontin at R333 and R339 (Pontin-me) was generated by Peptron. Each antibody used 1 μg...
An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
FLAG antibody (catalogue #F7425) was purchased from Sigma and used at a dilution of 1:5,000; BAF155 antibody (sc-10756) was purchased from Santa Cruz and used at a dilution of 1:1,000; MED12 antibody (ab70842) was purchased from Abcam (Cambridge, MA) and used at 1:1,000 ...
(ChIP–seq) using an anti-HA antibody at the early four-cell stage (LincGETChIP–seq) to explore the genome-wideLincGET-binding sites. The results showed thatLincGETindeed binds around theCarm1gene locus, tending to bind long terminal repeats (LTRs) (Extended Data Fig.5a). Generally, we ...
sigma aldrich(西格玛) | Invitrogen(英骏) | Millipore | Abcam | CST | Roche | peprotech | prospec | pierce | BD Pharmingen | BD Biocoat | R&D | BioVision | Vector | Enzo life | eBioscience | ABR | Amresco | Cayman Chemical | 脂蛋白 | Merck | Qiagen(凯杰) | Santa cruz(三塔抗体...