Chromatin immunoprecipitation The following antibodies were used to perform ChIP: anti-H3K27Me3 (Cell Signaling, Cat. No: 9733), anti-BAF155 (Santa Cruz, Cat. No: sc-9746), anti-RNA polymerase II (Santa Cruz, Cat. No: sc-899), or anti-EZH2 (Cell Signaling, Cat. No: 5246). An i...
To confirm that NFIB is methylated by CARM1 in the cells, we immunoprecipitated (IPed) NFIB from both CARM1 WT MEFs and CARM1 KO MEFs, and detected the methylation of NFIB by immunoblotting with the specific ADMA antibody (D4H5) (Fig. 1c). The ADMA antibody only detected IPed NFIB ...
An antibody array indicated that Erk1/2 (Thr202/Tyr204), PARS40 (Thr246), and GSK3β (Ser9) expression are affected by CARM1, and western blotting verified that CARM1 promotes OS cell proliferation via pGSK3β/β-catenin/cyclinD1 signaling. Accordingly, CARM1 is a crucial gene in ...
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(ab12162) and anti-H3R17me2 (ab8284) (Abcam); anti-acetyl-Histone H4 (06-866) (Millipore); anti-IgG (sc-2025) (Santa Cruz Biotechnology). The antibody specifically recognizing arginine methylated Pontin at R333 and R339 (Pontin-me) was generated by Peptron. Each antibody used 1 μg...
In addition, IRE1α inhibitor B-I09 synergizes with immune checkpoint blockade anti-PD1 antibody in an immunocompetent CARM1-expressing ovarian cancer model. Our data show that pharmacological inhibition of the IRE1α/XBP1s pathway alone or in combination with immune checkpoint blockade represents a...
(ChIP–seq) using an anti-HA antibody at the early four-cell stage (LincGETChIP–seq) to explore the genome-wideLincGET-binding sites. The results showed thatLincGETindeed binds around theCarm1gene locus, tending to bind long terminal repeats (LTRs) (Extended Data Fig.5a). Generally, we ...
Prior to immunoprecipitation, the antibodies were preincubated with protein A sepharose beads (Santa Cruz) overnight at 4 °C. The antibody-chromatin complexes were subsequently pulled down using protein A sepharose beads, followed by extensive washing with high salt, lithium chloride, and TE ...