prausnitzii–derived EVs increased the intestinal barrier permeability via TJs (ZO1 and OCLN) as well as PPAR-α, PPAR-γ and PPAR β/δ genes and their targeted gene (ANGPTL4) in the Caco-2 cell line. Accordingly, it is suggested that F. prausnitzii–derived EVs can be considered as a...
cell line, Caco-2, is a model for intestinal absorption. We found proliferative effects on Caco-2 cells at micromolar levels of these compounds, as monitored by [3H]-thymidine incorporation into DNA. Flow cytometry cell cycle analysis confirmed accumulation in S phase from 21% (control) to ...
Quercetin inhibits expression of inflammatory cytokines through attenuation of NF-kappaB and p38 MAPK in HMC-1 human mast cell line. Inflamm Res. 2007;56:210–5. Article CAS PubMed Google Scholar Mastuda H, Morikawa T, Ueda K, Managi H, Yoshikawa M. Structural requirements of flavonoids ...
2.3. Cell culture Cells from the Caco-2 (HTB-37) human colon carcinoma cell line were obtained from the American-Type Culture Collection (ATCC; LGC Promochem, Middlesex, UK). Cells were routinely cultured in 75 cm2 polystyrene culture flasks at 37 °C in humidified 5% CO2/95% laboratory ...
Trotter PJ, Storch J (1993) Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J Biol Chem 268:10017–10023 PubMed CAS Google Scholar Velísek J, Davídek J, Hajslová J, Kubelka V, Janícek G, Mánková B (1978) Chlorohydrins in protein hydrolysa...
Encapsulation with PLGA of I3C and radioiodination have been performed. Anticancerogenic effects of I3C and I3C-PLGA have been investigated utilizing in vitro methods on breast adenocarcinoma epithelial (MCF7), colon adenocarcinoma epithelial (Caco2), prostate carcinoma epithelial (PC3) cells. Charac...
2.5. Cell Culture: Caco-2 and HuTu-80 The human colon cancer cell line (Caco-2) was cultured in DMEM supplemented with FBS (10%),l-glutamine (2%) and penicillin/streptomycin (1%) at 37 °C and 5% CO2. Cells were grown in 12-well plates until reaching confluence, after approximately ...