The robust intestinal ecto-ATPase activity present in Caco-2 cells impairs measurements of medium ATP. We thus transfected c-Myc-tagged GPI-anchored plasma membrane luciferase (pmeLuc) to Caco2 cell monolayer to monitor ATP release at the cell surface. Confluent Caco-2 cell monolayers were ...
[关键词] Caco-2细胞单层模型;跨膜电阻(TEER)值;碱性磷酸酶; [中图分类号] R965 [文献标识码] A [文章编号] 1672-2809(2015)14-0033-04 Establishment of Caco-2 cell monolayer model SUN Xu1,2, LUO Yu-ping2 1 Beijing Key Laboratory, National Center for Safety Evaluation of Drugs, National ...
因此,L-色氨酸可以作为食物、肠道疾病或促进肠道TJ屏障的膳食补充剂的候选物。 原文链接 Dietary l-tryptophan alleviated LPS-induced intestinal barrier injury by regulating tight junctions in a Caco-2 cell monolayer model. by Me...
近期,国际权威期刊《Food and Chemical Toxicology》刊登了该团队题为“Regulatory effects of flavonoids luteolin on BDE-209-induced intestinal epithelial barrier damage in Caco-2 cell monolayer model”的文章。硕士研究生袁进文为第一...
2cellmonolayersinstudyingmechanism,uptake,transport andgastrointestinaltoxicityofxenobioticswerealsoexplained. Keywords:Caco一 2cells,invitrotest,gastrointestinaltoxicology,xenobiotics 人们传统利用整体动物实验或游离的小肠肠段来研究药 物和外源化学物在肠道的吸收和毒性.近些年来,随着相关 技术的发展,人们开始越来越多利...
Caco-2 Monolayer Assay Description Monolayers based onCaco-2 cells(a human colon carcinoma cell line) express a wide range of transporter proteins on its cell membranes, similar to those of intestinal endothelium cells (Siissalo S et al. 2007, Calcagno AM et al. 2006), thus this cell line...
Fig 2 Morphology of Caco-2 cell monolayer (×100) Full size|PPT slide 2.2 细胞生长曲线 由图3可见,Caco-2细胞接种后在早期生长迅速,A值升高,在后期A值逐渐降低,细胞生长曲线呈下降趋势。但接种不同细胞浓度的Caco-2细胞生长曲线有差别:细胞在低浓度(5×104·mL-1)接种时第15天生长达高峰,A值为0.905...
celldensityin standardproceduresfor21d1Themorphologicalandgrowthcharactersofthemonolayerwereexaminedwithopticalmicroscope,electronmi2 croscopeandcelldensitydetermination1Thepolarizationandcompactificationofthemonolayerwasdeterminedbythealkalinephosphataseac2 tivity,transepithelialelectricalresistance(TEER)andapical2to2basolateral...
Assessment of Caco2 cell monolayer integrity Before incubation, it is necessary to check the barrier integrity of Caco-2 cells, which is typically assessed using Transepithelial Electrical Resistance (TEER) or Lucifer Yellow Rejection Assay (%LY Leakage). ...
Caco-2细胞系来源于人的直肠癌，其结构和生化作用类似于人小肠上皮细胞，含有与小肠刷状缘上皮相关的酶系，与正常的成熟小肠上皮细胞在体外培育过程中出现逆向分化(反分化)不同，Caco-2细胞在传统的细胞培养条件下，生长在多孔的可渗透的聚碳酸酯膜上可达到融合并自发分化为肠上皮细胞，形成连续的单层(monolayer)...