Several of the cell lines could be partly differentiated by addition of synthetic or biological factors to the medium. However, one of them, Caco-2, was rather unique as it was able to differentiate spontaneously when reaching confluence. Caco-2 (Cancer coli-2) was established from a human ...
Caco-2 cell lines in drug discovery--an updated perspective. J Basic Clin Pharm. 2010;1(2):63-6.Kumar KK, Karnati S, Reddy MB, Chandramouli R. Caco-2 cell lines in drug discovery - an updated perspective. J Basic Clin Pharm 2010;1(2):63-9....
Although Caco-2 cells have been found to express a large number of enzymes and transporter proteins present in normal human intestinal epithelium, recent studies suggest that there are variations between gene expression profiles of transformed epithelial cell lines like Caco-2, HT29 and normal human ...
Several of the cell lines could be partly differentiated by addition of synthetic or biological factors to the medium. However, one of them, Caco-2, was rather unique as it was able to differentiate spontaneously when reaching confluence. Caco-2 (Cancer coli-2) was established from a human ...
(RSNaCaS-VA) were prepared and evaluated for their in-vitro bioaccessibility and in-vitro bioavailability of VA through Caco-2 cell lines.VA degraded under acidic conditions as the physiological pH during digestion in stomach was highly acidic (1.2-1.8)...
Due to this, a variety of cloned Caco–2 cell lines has been established, and described in the literature. This chapter will however, focus on describing how to handle and cultivate the original Caco–2 cell line as obtained from cell culture collections like American Type Culture Collection ...
Caco-2 cells express retinoic acid binding protein I and retinol binding protein II. REFERENCES 1. Fogh, J., Wright, W.C. and Loveless, J.D. 1977. Absence of HeLa cell con- tamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214. 2. Fogh, J....
近十几年来,国外已普遍采用组织细胞模型作为药物筛选的工具,其中包括Caco-2细胞(thehumancolonadenocarc-inomacelllines)单层模型。Caco-2细胞系来源于人类结肠癌及直肠癌,普通培养条件下即可在有孔的多聚碳酸酯膜上自发的分化为肠上皮细胞单层,因此可以模拟体内小肠上皮细胞层,然而体外试验得到的数据必定与体内试验有...
结论:所建立的Caco-2 细胞模型完整性、通透性与极性分化特征通过验证,可作为模拟小肠药物转运过程的体外模型。 关键词 Caco-2 细胞; 吸收,体外; 跨膜电阻值; 碱性磷酸酶; 渗透性 正文 人结肠腺癌细胞( the human colon adenocarcinomacell lines,Caco-2 细胞) 来源于人类结肠和直肠癌细胞,在体外培养过程中形成单...
人结直肠腺癌细胞(thehumancolonadenocarcinomacelllines,caco-2细胞)来源于人类结肠和直肠癌细胞;当长满时,caco-2细胞表现出特征性的肠上皮细胞分化。caco-2细胞表达维甲酸结合蛋白i和维甲酸结合蛋白ⅱ,并呈角质蛋白阳性。 caco-2细胞单层膜模型是当前研究药物对细胞作用的常用工具。目前的建模方式是根据实验目的将...