圣路易斯华盛顿大学医学院(Washington University in St. Louis, School of Medicine)尤忠胜教授团队在之前的研究工作中发现DNA复制压力会导致Ca2+从细胞器中释放,激活下游的CaMKK2和AMPK 蛋白激酶,从而抑制DNA外切酶EXO1,防止复制叉DNA...
研究表明,复制应激能引发细胞内Ca2+浓度上升,进而激活CaMKK2和AMPK。🔥AMPK通过磷酸化Exo1的S746位点,抑制其在染色质上的招募,从而保护受损的复制叉结构,维护基因组的稳定性。🛡️这一发现不仅揭示了复制应激诱导的钙信号转导机制,也为理解AMPK在复制应激中的角色提供了新线索。🔍然而,仍有许多问题待解,如...
CaMKK2/AMPKOxidative stressTelbivudine (LdT), a widely prescribed anti-hepatitis B virus (HBV) drug for the treatment of chronic Hepatitis B (CHB), causes adverse reactions ranging from creatine kinase (CK) elevation to myopathy. The purpose of this study was to explore the mechanism(s) of ...
的Ca2+通道蛋白打开,Ca2+从溶酶体内流入细胞溶胶,细胞质中的Ca2+浓度升高,激活钙调磷酸酶,钙调磷酸酶催化TFEB-P去磷酸化形成TFEB,TFEB进入细胞核进而促进Atg基因表达,Atg8-PE增多促进自噬维持细胞(和生物体)生命活动;同时Ca2+既可以直接活化AMPK酶,也可以通过先活化CaMKKβ继而激活AMPK,活化的AMPK通过抑制mTOR介导...
Bradykinin stimulates endothelial cell fatty acid oxidation by CaMKK-dependent activation of AMPK. Atherosclerosis 2008;200:28–36. Article CAS PubMed Google Scholar Woods A, Dickerson K, Heath R, Hong SP, Momcilovic M, Johnstone SR, et al. Ca2+/calmodulin-dependent protein kinase kinase-beta...
黄芪多糖通过AMPK介导的AS160磷酸化刺激葡萄糖摄取的机制研究 葡萄糖法检测细胞的葡萄糖摄取:(2)培养L6细胞,分别给予AMPK抑制齐Compound C (1μmol/L作用30min)和Ca2+/钙调蛋白依赖性蛋白激酶激酶p (CaMKKβ)选择性抑制(... 刘坚 ...
Activation of transient receptor potential vanilloid 4 protects articular cartilage against inflammatory responses via CaMKK/AMPK/NF-κB signaling pathway Article Open access 30 July 2021 TAP2, a peptide antagonist of Toll-like receptor 4, attenuates pain and cartilage degradation in a monoiodoacetate...
This study aimed to explore whether the neuroprotective mechanisms of PPF were linked to its regulation of Ca2+/CaMKKβ (calmodulin-dependent protein kinase kinase β)/AMPK (AMP-activated protein kinase)/mTOR (mammalian target of rapamycin)/autophagy pathway.关键词: Propofol...
It should be noted that the effect of STO-609 on ACC phosphorylation could be due to inhibition of both CaMKK and AMPK by STO-609 (see below). STO-609 was reported to be a specific inhibitor of CaMKKα and CaMKKβ, based on tests with seven other protein kinases (Tokumitsu et al....
(B) Bar graph representing the pAMPK:AMPK ratio from (A). n = 6. (C) Immunoblot analysis for phospho and total CaMKK-II. n = 6. (D) Hepatocyte lysates from AMPKα1/α2fl/fl and AMPKα1/α2fl/flCre+ mice were probed for AMPKα protein abundance. n = 3. (E) Hepatic TAG ...